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NEB/Monarch? Plasmid Miniprep Kit/T1010S/250 preps
  • NEB/Monarch? Plasmid Miniprep Kit/T1010S/250 preps

NEB/Monarch? Plasmid Miniprep Kit/T1010S/250 preps

價(jià)格: ￥4188.00 市場(chǎng)價(jià): 6980.00
貨號(hào): T1010S-250preps
品牌: NEB
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  • 詳情
  • 使用說(shuō)明
  • 常見問(wèn)題

    • Description:

      ?
      TheMonarchPlasmidMiniprepKitisarapidandreliablemethodforthepurificationofhighqualityplasmidDNA.ThismethodemploysstandardcellresUSPension,alkalinelysis,andneutralizationsteps,withtheadditionalbenefitofcolorindicatorsatcertainstepstoeasilymonitorcompletion.Uniquewashbuffersensuresalts,proteins,RNAandothercellularcomponentsareremoved,allowinglow-volumeelutionofconcentrated,highlypureDNA.Protocolsarefastanduserfriendly,savingyouvaluabletime.Elutioninaslittleas30μlprovidesconcentratedDNAforuseindownstreamapplications,suchasrestrictiondigests,DNAsequencing,PCRandotherenzymaticmanipulations.DesignedwithsustainABIlityinmind,MonarchkitsusesignificantlylessplasticandresponsIBLy-sourced,recyclablepackaging.

      Viewourvideosonprotocols,tips,andrecyclingMonarch.

      MonarchPlasmidMiniprepColumnDesign
      Monarch Plasmid Miniprep Column Design
      Advantages:
      • Eluteinaslittleas30μl
      • Preventbufferretentionandsaltcarry-overwithoptimizedcolumndesign
      • Reducehandsontimewithfasterprotocolsandlessspintime
      • Monitorcompletionofcertainstepsusingcoloredbuffersystem
      • NoneedtoaddRNasebeforestarting
      • Easilylabelcolumnsusingtabandfrostedsurfaces
      • Buffersandcolumnsavailableseparately
      • Significantlylessplasticusedwhencomparedwithotherkits
      • Responsibly-sourcedandrecyclablepackaging
      • Nohazardousmaterialsfees?

      Specifications
      CultureVolume:1–5ml
      BindingCapacity:upto20μg
      PlasmidSize:upto25kb
      TypicalRecovery:upto20μg.Yielddependsonplasmidcopynumber,host
      strain,culturevolume,andgrowthconditions.
      ElutionVolume:≥30μl
      Purity:A260/280andA260/230≥1.8
      ProtocolTime:10?minutesofspinandincubationtime
      Compatible
      Downstream
      Applications:      		restrictiondigestionandotherenzymaticmanipulations,
      transformation,transfection,DNAsequencing,PCR,labeling,
      cell-freeproteinsynthesis,etc.


      MonarchPlasmidMiniprepKitProtocol?
      Monarch Plasmid Miniprep Kit Protocol

      MonarchPlasmidMiniprepKitsconsistentlyproducemoreconcentratedplasmidDNAwithequivalentyield,purityandfunctionalityascomparedtotheleADIngsupplier?
      Monarch Plasmid Miniprep Kits consistently produce more concentrated plasmid DNA with equivalent yield, purity and functionality as compared to the leading supplier. Preps were performed according to recommended protocols using 1.5 ml aliquots of the same overnight culture. One microliter of each prep was digested with HindIII-HF (NEB #R3104) to linearize the vector and the digests were resolved on a 1% w/v agarose gel.
      Prepswereperformedaccordingtorecommendedprotocolsusing1.5mlaliquotsofthesameovernightculture.OnemicroliterofeachprepwasdigestedwithHindIII-HF(NEB#R3104)tolinearizethevectorandthedigestswereresolvedona1%w/vagarosegel.?

      PlasmidDNApurifiedusingtheMonarchPlasmidMiniprepKitproducestransfectionefficienciesequivalenttoorbetterthanplasmidDNApurifiedusingtheQiagenQIAprep?SpinMiniprepKit?
      Plasmid DNA purified using the Monarch Plasmid Miniprep Kit produces transfection efficiencies equivalent to or better than plasmid DNA purified using the Qiagen QIAprep? Spin Miniprep Kit. Plasmid DNA encoding constitutively expressed GFP (pEGFP-C2) was prepared using either Monarch Plasmid Miniprep Kit or Qiagen QIAprep Spin Miniprep Kit. Four different cell lines (Cos-7, HEK293, HeLa, and Huh-7) were grown to 80-90% confluence and transfected with 100 ng of each plasmid, in complex with 0.3 μl Lipofectamine 2000, and 10 μl Opti-MEM. Five replicates for each cell type were performed using both DNA preps. GFP expressing cells were counted by flow cytometry 48 hrs post-transfection with a minimum of 2000 events collected per well. Average percentage of cells expressing GFP from all replicates is graphed and used as a measure of transfection efficiency.
      PlasmidDNAencodingconstitutivelyexpressedGFP(pEGFP-C2)waspreparedusingeitherMonarchPlasmidMiniprepKitorQiagenQIAprepSpinMiniprepKit.Fourdifferentcelllines(Cos-7,HEK293,HeLa,andHuh-7)weregrownto80-90%confluenceandtransfectedwith100ngofeachplasmid,incomplexwith0.3μlLipofectamine2000,and10μlOpti-MEM.FivereplicatesforeachcelltypewereperformedusingbothDNApreps.GFPexpressingcellswerecountedbyflowcytometry48hrspost-transfectionwithaminimumof2000eventscollectedperwell.AveragepercentageofcellsexpressingGFPfromallreplicatesisgraphedandusedasameasureoftransfectionefficiency.?

      DNAfromMonarchPlasmidMiniprepKitisreproducibly?compatiblewithDNAsequencing.
      DNA from Monarch Plasmid Miniprep Kit is reproducibly compatible with DNA sequencing. Plasmid DNA from three separate preps was sequenced using BigDye? Terminator chemistry on an Applied Biosystems 3730XL DNA Analyzer. The electropherograms demonstrate the quality of the DNA is reproducible.
      PlasmidDNAfromthreeseparateprepswassequencedusingBigDye?TerminatorchemistryonanApplied?Biosystems3730XLDNAAnalyzer.TheelectropherogramsdemonstratethequalityoftheDNAisreproducible.

      LearnMoreAboutMonarch
      • NEBMonarch.com
      • Optimizeyourresultswithouruniquecolumndesign
      • EnhanceyourDNApurificationexperience
      • FeelgoodaboutchoosingMonarch
      • ChooseMonarchkitsforpurevalue

      KitComponents

      Thefollowingreagentsaresuppliedwiththisproduct:

      Storeat(°C)Concentration
      Monarch®PlasmidResuspensionBuffer(B1)251X
      Monarch®PlasmidLysisBuffer(B2)251X
      Monarch® PlasmidNeutralizationBuffer(B3)41X
      Monarch®PlasmidWashBuffer1251X
      Monarch®PlasmidWashBuffer2255X
      Monarch®DNAElutionBuffer251X
      Monarch®PlasmidMiniprepColumns25

      Notes:

      Thekitshouldbestoredatroomtemperature.Alwayskeepbufferbottlestightlyclosedandkeepcolumnssealedintheenclosedzip-lockbag.AfterPlasmidNeutralizationBuffer(B3)isopened,itshouldbestoredat4°C.Forinformationregardingthecompositionofbuffers,pleaseconsulttheSafetyDataSheets.Properlaboratorysafetypracticesshouldbeemployed,includingtheuseoflabcoats,gloves,andeyeprotection.
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