ApplicationData | DetectionofhumanGSK-3β,phosphorylatedatTyr216byimmunoblotting.Samples:Wholecelllysatefromcontrolcells(lane1)orcontrollysatespikedwithhumanrecombinantGSK-3β(lanes2-5).Primaryantibody:PhosphoDetect?Anti-GlycogenSynthaseKinase-3α/β(pTyr279/216)RabbitpAb(Cat.No.ST1013)(1:1000)pre-incubatedwithoutpeptide(lanes1,5),orwithphosphopeptideimmunogen(lane2),agenericphosphoserine-containingpeptide(lane3),ornon-phosphopeptidecorrespondingtotheimmunogen(lane4).Detection:chemiluminescence. |