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NEB/SNAP-Capture Magnetic Beads/S9145S/2 ml
  • NEB/SNAP-Capture Magnetic Beads/S9145S/2 ml

NEB/SNAP-Capture Magnetic Beads/S9145S/2 ml

價格: ¥2460.00 市場價: 4100.00

貨號: S9145S-2ml
品牌: NEB
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    • Description:

      TheSNAP-CaptureMagneticBeadsareusedtoselectivelyimmobilizeandmagneticallyseparateaSNAP-tag?fusionproteinfromsolutionusingmagneticagarosebeads.Thesebeadsshowalownon-specificabsorptionofproteinsfromacomplexlysate,makingthemsuitableforpulldownapplications.TheSNAP-CaptureMagneticBeadsarepreparedbythecouplingofSNAP-tagsubstratebenzylguaninewithhighlystable75-150μmsuperparamagneticparticles.TwomlofSNAP-CaptureMagneticBeadsissufficienttoperform25immobilizationassaysusing80μlofbeadsUSPensionperassay.

      TheSNAP-tagisanoveltoolforproteinresearch,allowingthespecific,covalentattachmentofvirtuallyanymoleculeormodifiedsurfacetoaproteinofinterest.TheSNAP-tagisaproteinbasedonmammalianO6-alkylguanine-DNAalkyltransferase(AGT).?SNAP-tagsubstratesarederivativesofbenzylpurinesandbenzylpyrimidines.?Intheimmobilizationreaction,theSNAP-tagiscovalentlyattachedtothesubstitutedbenzylgroupoftheSNAP-CaptureMagneticBeads.

      Therearetwostepstousingthissystem:sub-cloningandexpressionoftheproteinofinterestasaSNAP-tagfusion,andcaptureandimmobilizationofthefusionproteinusingSNAP-CaptureMagneticBeads.

      Figure1:SubstratestructureonSNAP-CaptureMagneticBeads?
      Figure 1: Substrate structure on SNAP-Capture Magnetic Beads

      Notes:

      StorageofMagneticBeads:DonotfreezetheSNAP-CaptureMagneticBeads.Storetheunusedbeadsat4°C.Withproperstorage,beadsshouldbestableforatleasttwoyears.CorrectstorageandhandlingofSNAP-tagfusionproteinsisessentialtomaintainreactivityoftheSNAP-tagpriortoimmobilization.SNAP-tagfusionproteinscanbepurifiedbeforeimmobilization,buttheimmobilizationreactionalsoworksinnon-purifiedproteinsolutionsincludingcelllysates.?Add1mMDTTtobuffersusedforthestorageofSNAP-tagfusionproteins.Proteinsamplesshouldbestoredat-20°C,orat-80°Cforlong-termstorage.Handlingattemperaturesabove0°CshouldbeminimizedbythawingtheSNAP-tagproteinsamplesshortlybeforeuse,andkeepingthemoniceuntiljustbeforetheimmobilization.Ifaparticularfusionproteinrequiresbufferswithoutreducingagents,minimizehandlingstepsoftheproteinabove4°Cbeforethelabelingreaction.TheSNAP-tagitselfistolerantofawiderangeofbuffers.Therequirementsofyourfusionpartnershoulddictatetheselectionofthebuffer.Thefollowingstoragebuffercompositionisrecommended,especiallywhenfreezingproteinmaterial:pHbetween7.0and8.0,monovalentsalts(e.g.sodiumchloride)between50mMand250mM,andatleast1mMDTT.Non-ionicdetergentscanbeaddedifrequired,butionicdetergentsshouldbeavoidedbecausetheyreducetheactivityoftheSNAP-tag.Manyproteinsbenefitfromtheadditionofglycerolforfrozenstorage,typically20%v/v.ImmobilizedSNAP-tagfusionproteinsshouldbestoredat4°C.Sodiumazidemaybeaddedto2mMfinalconcentrationtopreventbacterialgrowth.DependingonthestABIlityofthefusionpartner,undertheseconditionstheimmobilizedproteinshouldbestableat2–6°Cforseveralmonths.TheSNAP-CaptureMagneticBeadsshouldnotbefrozen.TheSNAP-tagfusionproteinislinkedtotheSNAP-CaptureMagneticBeadsbyacovalentbond.ThereforetheimmobilizedproteinisessentiallyirreversIBLyboundtothebeads.ItisimportanthowevertopreservethefunctionalstabilityoftheproteinfusedtotheSNAP-tagasmuchaspossible.Werecommendhandlingtheimmobilizedfusionproteinandstoringbetweenuseat4°C,toprepareitjustbeforeuse,andtohandleitasgentlyaspossible.
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