Rabbit polyclonal antibody raised against synthetic peptide of ALK.
Immunogen:
A synthetic peptide corresponding to amino acids 1570-1619 of human ALK.
Host:
Rabbit
Theoretical MW (kDa):
176
Reactivity:
Human
Specificity:
This antibody detects endogenous levels of total ALK protein.
Form:
Liquid
Recommend Usage:
ELISA (1:1000)Immunofluorescence (1:100~1:500)Immunohistochemistry (1:50~1:100)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS ((without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
The oncoprotein NPM-ALK of anaplastic large-cell lymphoma induces JUNB transcription via ERK1/2 and JunB translation via mTOR signaling.Staber PB, Vesely P, Haq N, Ott RG, Funato K, Bambach I, Fuchs C, Schauer S, Linkesch W, Hrzenjak A, Dirks WG, Sexl V, Bergler H, Kadin ME, Sternberg DW, Kenner L, Hoefler G.Blood. 2007 Nov 1;110(9):3374-83. Epub 2007 Aug 9.
2.
Nucleophosmin/anaplastic lymphoma kinase (NPM/ALK) oncoprotein induces the T regulatory cell phenotype by activating STAT3.Kasprzycka M, Marzec M, Liu X, Zhang Q, Wasik MA.Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):9964-9. Epub 2006 Jun 9.
3.
Prognostic significance of CD56 expression for ALK-positive and ALK-negative anaplastic large-cell lymphoma of T/null cell phenotype.Suzuki R, Kagami Y, Takeuchi K, Kami M, Okamoto M, Ichinohasama R, Mori N, Kojima M, Yoshino T, Yamabe H, Shiota M, Mori S, Ogura M, Hamajima N, Seto M, Suchi T, Morishima Y, Nakamura S.Blood. 2000 Nov 1;96(9):2993-3000.
4.
Lymphomas expressing ALK fusion protein(s) other than NPM-ALK.Falini B, Pulford K, Pucciarini A, Carbone A, De Wolf-Peeters C, Cordell J, Fizzotti M, Santucci A, Pelicci PG, Pileri S, Campo E, Ott G, Delsol G, Mason DY.Blood. 1999 Nov 15;94(10):3509-15.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ALK polyclonal antibody (Cat # PAB18081).Peptide "+" means "with peptide blocking".
Immunohistochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells, using ALK polyclonal antibody (Cat # PAB18081).Peptide "+" means "with peptide blocking".
The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3" half of ALK, derived from chromosome 2, is fused to the 5" portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK (leukocyte tyrosine kinase). ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. [provided by RefSeq
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