Rabbit polyclonal antibody raised against partial recombinant ACTB.
Immunogen:
Recombinant protein corresponding to bacteria ACTB.
Host:
Rabbit
Reactivity:
Human
Form:
Liquid
Recommend Usage:
Western Blot (1:1000-1:4000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction:
Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Cell-surface actin binds plasminogen and modulates neurotransmitter release from catecholaminergic cells.Miles LA, Andronicos NM, Baik N, Parmer RJ.J Neurosci. 2006 Dec 13;26(50):13017-24.
Applications
Western Blot (Cell lysate)
Western blot analysis of 5 ug extracts from HeLa, 293T, MCF-7 cells (Lane 1, 2, 3) using ACTB polyclonal antibody (Cat # PAB17012).