Rabbit polyclonal antibody raised against synthetic peptide of MAPKAP Kinase 2.
Immunogen:
A synthetic peptide (conjugated with KLH) corresponding to amino acids 310-325 of rabbit MAPKAP Kinase 2.
Sequence:
MTSALATMRVDYEQIK
Host:
Rabbit
Reactivity:
Chicken, Clawed frog, Hamster, Human, Mouse, Rabbit, Rat
Specificity:
This polyclonal anti-MAPKAP Kinase 2 antibody recognizes native and over-expressed protein found in various tissues and extracts.
Form:
Liquid
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Recommend Usage:
ELISA (1:15000)Western Blot (1:2000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
Download
Publication Reference
1.
Retaining of the assembly capability of vimentin phosphorylated by mitogen-activated protein kinase-activated protein kinase-2.Cheng TJ, Tseng YF, Chang WM, Chang MD, Lai YK.J Cell Biochem. 2003 Jun 1;89(3):589-602.
2.
Inhibition of SAPK2a/p38 prevents hnRNP A0 phosphorylation by MAPKAP-K2 and its interaction with cytokine mRNAs.Rousseau S, Morrice N, Peggie M, Campbell DG, Gaestel M, Cohen P.EMBO J. 2002 Dec 2;21(23):6505-14.
3.
MAPKAP kinase 2 is essential for LPS-induced TNF-alpha biosynthesis.Kotlyarov A, Neininger A, Schubert C, Eckert R, Birchmeier C, Volk HD, Gaestel M.Nat Cell Biol. 1999 Jun;1(2):94-7.
Applications
Western Blot (Cell lysate)
Affinity purified MAPKAP Kinase 2 polyclonal antibody (Cat # PAB10263) detects MAPKAP Kinase 2 in unstimulated human HeLa whole cell lysate by western blot.MAPKAP Kinase 2 polyclonal antibody used at a 1 : 2000 dilution to detect 20 ug of whole cell lysate containing MAPKAP Kinase 2.This antibody detects a single 44 KDa protein as indicated in crude extracts prepared from either unstimulatedor TNFalpha stimulated human HeLa cell lysates.A 4-20% gradient gel was used to separate the protein by SDS-PAGE.The protein was transferred to nitrocellulose using standard methods.After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.LICOR"s Odyssey® Infrared Imaging System was used to scan and process the image.
購物車為空，快去購物吧！
4000-520-616
咨詢顧問
特別 提示
Download
