The antiserum was produced against synthesized phospho-peptide derived from human MAP2K7 around the phosphorylation site of Ser271.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
MAP2K7 (Phospho-Ser271) Antibody detects endogenous levels of MAP2K7 only when phosphorylated at Ser271.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAP2K7
Database Links
Gene ID: 5609 Swiss-Prot #: O14733
OMIM #: 603014 Unigene #: Hs.531754
Target Modification
Phospho
Modification Sites
H:S271, M:S287, R:S271
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAP2K7 (Phospho-Ser271) Antibody
Image 2
Western blot analysis of lysates from NIH/3T3 cells treated with insulin 0.01U/ml 15' and NIH/3T3 cells treated with EGF 200ng/ml 30', using MAP2K7 (Phospho-Ser271) Antibody.