Description:
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Peptide:N-GlycosidaseF,alsoknownasPNGaseF,isanamidasewhichissuppliedglycerolfreeforoptimalperformanceinHPLCintensivemethods.PNGaseFcleavesbetweentheinnermostGlcNAcandasparagineresiduesofhighmannose,hybridandcomplexoligosaccharidesfromN-linkedglycoproteins(1).
ProductSource
PNGaseFispurifiedfromFlavobacteriummeningosepticum(3),freeofcontaminants(EndoF,proteases,etc.).ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
GlycoproteinDenaturingBuffer | -20 | 10X |
NP-40 | -20 | 10% |
GlycoBuffer2 | -20 | 10X |
Notes:
SincePNGaseFactivityisinhibitedbySDS,itisessentialtohaveNP-40presentinthereactionmixture.Whythisnon-ionicdetergentcounteractstheSDSinhibitionisunknownatpresent.Todeglycosylateanativeglycoprotein,longerincubationtimeaswellasmoreenzymemayberequired.?PNGaseFwillnotcleaveN-linkedglycanscontainingcoreα1-3Fucose.?Repeatedfreezethawcyclesdegradeenzymeactivityovertime.?Typicalreactionconditions:PleaseseeProtocoltab