ApplicationReferences | ParaffinSectionsPezzella,F.,etal.1994.J.Clin.Pathol.47,592.OriginalCloneGannon,J.V.,etal.1990.EMBOJ.9,1595.EpitopeIdentificationStephen,C.W.andLane,D.P.,1992.J.Mol.Biol.225,1.ImmunofluorescenceBernardi,R.,etal.2004.Nat.CellBiol.6,665. |
KeyApplications | Immunoblotting(WesternBlotting) Immunofluorescence Immunoprecipitation ParaffinSections FlowCytometry FrozenSections GelShift
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ApplicationNotes | FlowCytometry(1-20μg/ml) FrozenSections(10μg/ml) GelShift(seecomments) Immunoblotting(5μg/ml) Immunofluorescence(1-20μg/ml) Immunoprecipitation(1μgpersample) ParaffinSections(seeapplicationreferences) |
ApplicationComments | Undernon-denaturingconditions(immunoprecipitation,immunofluorescenceandfrozensections),p53(Ab-3)doesnotbindtonormal(wild-type)p53protein;itrecognizesanepitopeexposedbyactivatingmutationsordenaturation.Indenaturingprotocols(immunoblottingandparaffinsections),p53(Ab-3)willrecognizebothmutantandwild-typep53.Willnotrecognizetosomep53moleculeswithmutationsintheRHSVVepitope,butwillrecognizeTFIIIA,whichhastheRHSVVepitope.Forgelshiftassay,resUSPendin100μlbuffer.Antibodiesshouldbetitratedforoptimalresultsinindividualsystems. |