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Millipore/NS145 | Dopaminergic Neuron Integration and Characterization Kit/NS145/1 kit
  • Millipore/NS145 | Dopaminergic Neuron Integration and Characterization Kit/NS145/1 kit

Millipore/NS145 | Dopaminergic Neuron Integration and Characterization Kit/NS145/1 kit

價格: ￥11076.00 市場價: 18460.00
貨號: NS145
品牌: Millipore
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    • Description
      CatalogueNumberNS145
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionDopaminergicNeuronIntegrationandCharacterizationKit
      OverviewSeeindividualdatasheetsforspecificcomponentdescriptions.
      BackgroundInformationDopaminergicneurondysfunctionandlosscorrelateswithnumerousprogressiveanddevastatingneurologicaldiseases.Stemcellresearchandapplicationsarerapidlymovingtowardtheintroductionandsubsequentpost-developmentanalysisofembryonicstem(ES)cell-deriveddopaminergicneuronsinadulttissue.TheNS145ispartofanexpandingChemicon/UpstateproductlinetargetingdevelopmentandplasticityinneuroBIOLOGy.NS145isanovel,broadlyencompassingkitforthelocalization,characterizationandanalysisofEScellderiveddopaminergicneuronintegrationintoadultcentralnervoussystem(CNS)tissue.Thiskitallowsresearchersto:(1)identifyandcharacterizedopaminergicneuronsintissue;and(2)identifyotherneighboringneuronaltypesandastrocytes.Inaddition,theantibodiesareamixtureofmouseandratmonoclonalsandrABItandgoatpolyclonalstooptimizedouble,triple,andquadruplestainingparADIgms.Thekitcomponentbreakdownisasfollows:

      (1)Identifyingdopaminergicneurons.ResearchersareinterestedinidentifyingthesubtypeofneuronthattheirtaggedEShavematuredintofollowingintroductionandintegrationintotherecipientCNS.Thisisakeystepindopaminergicneuronreplacementtherapy.ThiskitincludesantibodiesfortheidentificationofdopaminergicneuronsusingseveraldiverseMarkerstargetingdifferentstagesintheneurotransmittersynthesisandmaintainance:DopaDecarboxylase(AB1569-50UL);TyrosineHydroxylase(TH;MAB5280-20UG);DopamineTransporter(DAT;MAB369-50UL).

      (2)Identifyotherneuronalsubtypesandastrocytesneighboringthetransplantedcells.InordertomeasurethelevelofstructuralintegrationofthetaggedEScells,researchersoftenusegeneralmarkersofneuronalsubtypeandastrocytestocharacterizethelocalenvironmentinwhichtheEScell-deriveddopaminergicneuronsareintegrating.Thekitincludesantibodiesforthegeneralidentificationofneurons(NeuN;MAB377-100UG)andastrocytes(GFAP;AB5804).Alsoincludedareexcellentmarkersfortheidentificationofcholinergic(ChAT;AB144P-200UL)andSEROtonergic(Serotonin;MAB352-50UL)neurons.

      Additional,individualreagentscanbeorderedseparatelyinlargerquantities.
      ProductInformation
      Components
      • Onekitcontainingthefollowingcomponents:
      • 100μgofNeuNmousemonoclonalMAB377-100UG
      • 50μLofDopaDecarboxylaserabbitpolyclonalAB1569-50UL
      • 50μLofDATratmonoclonalMAB369-50UL
      • 50μLofGFAPrabbitpolyclonalAB5804
      • 200μLofChATgoatpolyclonalAB144P-200UL
      • 50μLofSerotoninratmonoclonalMAB352-50UL
      • 20μgofTyrosineHydroxylasemousemonoclonalMAB5280-20UG
      StorageandShippingInformation
      StorageConditionsMaintaincomponentsaccordingtotheindividualdatasheets.

      ImportantNote:Duringshipment,smallvolumesofproductwilloccasionallybecomeentrappedinthesealoftheproductvial.Forproductswithvolumesof200μLorless,werecommendgentlytappingthevialonahardsurfaceorbrieflycentrifugingthevialinatable-topcentrifugetodislodgeanyliquidinthecontainer"scap.
      Applications
      KeyApplications
      • Immunohistochemistry
      • Immunocytochemistry
      BiologicalInformation
      SpeciesReactivity
      • Vertebrates
      EntrezGeneNumber
      EntrezGeneSummaryThedopaminetransporter(DAT1)mediatestheactivereuptakeofdopaminefromthesynapseandisaprincipalregulatorofdopaminergicneurotransmission.TheDAT1genehasbeenimplicatedinhumandisorderssuchasparkinsonism,Tourettesyndrome,andsubstanceabuse(Vandenberghetal.,1992[PubMed1359373]).[suppliedbyOMIM]
      GeneSymbol
      • DDC
      • AADC
      • EC4.1.1.28
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q01959#Aminetransporter.Terminatestheactionofdopaminebyitshighaffinitysodium-dependentreuptakeintopresynapticterminals.
      SIZE:620aminoacids;68495Da
      SUBUNIT:Homooligomer;disulfide-linked.InteractswithPRKCABPandTGFB1I1.
      SUBCELLULARLOCATION:Membrane;Multi-passmembraneprotein.
      SIMILARITY:SwissProt:Q01959##Belongstothesodium:neurotransmittersymporter(SNF)family.
      MISCELLANEOUS:Thisproteinisthetargetofpsychomotorstimulantssuchasamphetaminesorcocaine.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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