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Millipore/MTH17MAG-47K | MILLIPLEX <small>MAP</small> Mouse TH17 Magnetic Bead Panel - Immunology Multiplex Assay/MTH17M
  • Millipore/MTH17MAG-47K | MILLIPLEX <small>MAP</small> Mouse TH17 Magnetic Bead Panel - Immunology Multiplex Assay/MTH17M

Millipore/MTH17MAG-47K | MILLIPLEX MAP Mouse TH17 Magnetic Bead Panel - Immunology Multiplex Assay/MTH17M

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貨號: MTH17MAG-47K
品牌: Millipore
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    • Description
      CatalogueNumberMTH17MAG-47K
      TradeName
      • MILLIPLEX
      DescriptionMILLIPLEXMAPMouseTH17MagneticBeadPanel-ImmunologyMultiplexAssay
      OverviewTheMILLIPLEXMAPMouseTh17MagneticBeadpanelenablesyoutofocusonthetherapeuticpotentialofthemodulationofCD4T-helpercellcytokines.CoupledwiththeLuminex?xMAP?platforminamagneticbeadformat,youreceivetheadvantageofidealspeedandsensitivity,allowingquantitativemultiplexdetectionofdozensofanalytessimultaneously,whichcandramaticallyimproveproductivity.
      BackgroundInformationCD4T-helpercellsaremajorplayersinadaptiveimmunity.Basedontheirexpressionprofileoftranscriptionfactorsandsecretedcytokines,thesecellsaredividedintothemajorsubsetsTh1,Th2,Th17andTregulatory(Treg)cells.Th1cells,inducedbyIL-12andenhancedbyIFN?,secreteIFN?thatactivatesmacrophages.InducedbyIL-4,Th2cellsprimarilyproduceIL-4,IL-5,IL-13,IL-25andIL-17E,playingaroleinallergy.Tregcells,inducedbyTGFβ,producetheanti-inflammatorycytokinesIL-10andTGFβandfunctiontocontrolT-cellresponsestopreventautoimmunereactions.Th17cells,amorerecentlydiscoveredsubsetcharacterizedbytheproductionofIL-17,areinducedbyTGFβcombinedwithIL-23andIL-1βinhumans,andTGFβ,IL-6andIL-21inmice.ActivatedTh17cellssecreteIL-17A,IL-17F,IL-21,IL-22andTNFαtopromotetissueinflammation.

      Th17cellsareinvolvedintheclearanceofextracellularbacteriaandfungi.TheyareabundantintheintestinallaminapropriaandfunctionasabarrieragainstinvADIngpathogens.ExcessiveamountsofTh17cellshavebeenimplicatedinthepathogenesisofseveralautoimmunediseases,includingmultiplesclerosis,psoriasis,juvenilediabetes,rheumatoidarthritis,Crohn"sdiseaseandautoimmuneuveitis.InadditionTh17cellsplayanimportantroleintumordevelopment,progressionandmetastasis,potentiallyinbothpromotingandinhibitingtumorgrowth.
      ProductInformation
      DetectionmethodLuminexxMAP
      ConfigurationDesignyourmultiplexkitbychoosingavailableanalyteswithinthispanel.
      Precision,%
      • Inter-assay:<>
      • Intra-assay:<>
      PanelTypeMAGNETICCytokines/Chemokines
      StorageandShippingInformation
      StorageConditionsRecommendedstorageforkitcomponentsis2-8°C.
      Applications
      ApplicationSimultaneouslyanalyzemultipleTh17cytokineandchemokinebioMarkerswiththeTh17Bead-BasedMultiplexAssaysusingtheLuminextechnology,inhumanserum,plasmaandcellculturesamples.
      KeyApplications
      • Multiplexing
      ApplicationNotes?Thisisatwo-hourorovernightincubationassayrequiring25μLofneatplasmaorserum,or25μLcellculturesupernatantperwell.
      ?ThiskitmaybeusedfortheanalysisofalloranycombinationoftheTh17panelanalytesinserum,plasmaortissue/celllysateandculturesupernatant
      BIOLOGicalInformation
      SpeciesReactivity
      • Mouse
      AnalytesAvailable
      • CD40Ligand
      • GM-CSF
      • IFN-γ
      • IL-1β
      • IL-2
      • IL-4
      • IL-5
      • IL-6
      • IL-10
      • IL-12(p70)
      • IL-13
      • IL-15
      • IL-17A
      • IL-17E/IL-25
      • IL-17F
      • IL-21
      • IL-22
      • IL-23
      • IL-27
      • IL-28B
      • IL-31
      • IL-33
      • MIP-3α/CCL20
      • TNF-α
      • TNFβ
      CrossReactivityTherewasnoorminimumcross-reactivitybetweentheantibodiesandanyotheranalytesinthepanel(seedetailsinassayprotocol).
      PhysicochemicalInformation
      Sensitivity
      • Refertokitprotocolforsensitivitiesofindividualbiomarkers.
      LinearityofDilution1:4111.0%1:8113.1%
      StandardCurveRange
      • CD40LIL-31&MIP-3α:49–50,000pg/mL
      • GM-CSF&IL-15:34–35,000pg/mL
      • IFNγ&IL-6:7.8–8,000pg/mL
      • IL-1β:15–15,000pg/mL
      • IL-2:6.9–6,000pg/mL
      • IL-4:1.5–1,500pg/mL
      • IL-5:4.9–5,000pg/mL
      • IL-10,IL-12(p70),&IL-21:20–20,000pg/mL
      • IL-13&IL-17A:39–40,000pg/mL
      • IL-17E/IL-25:586–600,000pg/mL
      • IL-17F:10–10,000pg/mL
      • IL-22:2.4–2,500pg/mL
      • IL-23:342-350,000pg/mL
      • IL-27:879–900,000pg/mL
      • IL-28B:127–130,000pg/mL
      • IL-33:78–80,000pg/mL
      • TNFβ:488–500,000pg/mL
      • TNFα:3.4–3,500pg/mL
      Dimensions
      MaterialsInformation
      MaterialsInformation
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