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Millipore/MABE197 | Anti-phospho-Retinoblastoma (Thr821) Antibody, clone E231, rabbit monoclonal/MABE197/100 µL
  • Millipore/MABE197 | Anti-phospho-Retinoblastoma (Thr821) Antibody, clone E231, rabbit monoclonal/MABE197/100 µL

Millipore/MABE197 | Anti-phospho-Retinoblastoma (Thr821) Antibody, clone E231, rabbit monoclonal/MABE197/100 µL

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貨號: MABE197
品牌: Millipore
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    • Description
      CatalogueNumberMABE197
      DescriptionAnti-phospho-Retinoblastoma(Thr821)Antibody,cloneE231,rabbitmonoclonal
      AlternateNames
      • retinoblastomasUSPectibilityprotein
      • retinoblastoma-associatedprotein
      BackgroundInformationRetinoblastomatumorsuppressorprotein(Rb)isassociatedwithDNAbindingactivityandisthoughttoplayasignificantroleincontrollingcellcycleprogressionduringtumorgrowth(1,2).Cellcycle-dependentphosphorylationbycdk’sinhibitsRbbinding,thusallowingcellcycleprogression(3).RbinactivationandcellcycleprogressionlikelyrequiresfirstphosphorylationbycyclinDcdk4/6followedbycyclinE-cdk2phosphorylation(4).
      ProductInformation
      FormatDilutedCultureSupernatant
      Control
      • PhosphataseuntreatedandtreatedJurkatcelllysates
      PresentationRabbitMonoclonalinbuffercontaining50mMTris-Glycine(pH7.4),0.15MNaClcontaining40%Glycerol,0.01%sodiumazideand0.05%BSA.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20oCfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow
      -20°CmaycauseglycerolcontainingSolutionstobecomefrozenduringstorage.
      Applications
      ApplicationDetectphospho-RetinoblastomawithAnti-phospho-Retinoblastoma(Thr821)Antibody,cloneE231(RabbitMonoclonalAntibody),thathasbeenshowntoworkinWB,ICC&IP.
      KeyApplications
      • WesternBlotting
      • Immunocytochemistry
      • Immunoprecipitation
      ApplicationNotesImmunoprecipitationAnalysis:1:20dilutionfromarepresentativelotwasusedinIP.
      ImmunocytochemistryAnalysis:1:100-1:250dilutionfromarepresentativelotdetectedRbinHeLacells.
      BIOLOGicalInformation
      ImmunogenSyntheticphospho-peptidecorrespondingtohumanRbphosphorylatedatThr821.
      EpitopePhosphorylatedThr821
      CloneE231
      HostRabbit
      IsotypeIgG
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      GeneSymbol
      • retinoblastoma1
      • RB
      • p105-Rb
      • pp110
      • pRb
      • osteosarcoma
      • OSRC
      • Rb
      Modifications
      • Phosphorylation
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:Keyregulatorofentryintocelldivisionthatactsasatumorsuppressor.ActsasatranscriptionrepressorofE2F1targetgenes.Theunderphosphorylated,activeformofRB1interactswithE2F1andrepressesitstranscriptionactivity,leADIngtocellcyclearrest.Directlyinvolvedinheterochromatinformationbymaintainingoverallchromatinstructureand,inparticular,thatofconstitutiveheterochromatinbystabilizinghistonemethylation.RecruitsandtargetshistonemethyltransferasesSUV39H1,SUV420H1andSUV420H2,leadingtoepigenetictranscriptionalrepression.ControlshistoneH4"Lys-20"trimethylation.InhibitstheintrinsickinaseactivityofTAF1.MediatestranscriptionalrepressionbySMARCA4/BRG1byrecruitingahistonedeacetylase(HDAC)complextothec-FOSpromoter.Inrestingneurons,transcriptionofthec-FOSpromoterisinhibitedbyBRG1-dependentrecruitmentofaphospho-RB1-HDAC1repressorcomplex.Uponcalciuminflux,RB1isdephosphorylatedbycalcineurin,whichleadstoreleaseoftherepressorcomplexBysimilarity.Incaseofviralinfections,interactionswithSV40largeTantigen,HPVE7proteinoradenovirusE1AproteininducethedisassemblyofRB1-E2F1complextherebydisruptingRB1"sactivity.
      SUBUNITSTRUCTURE:InteractswithATAD5.InteractswithPRMT2Bysimilarity.ThehypophosphorylatedforminteractswithandsequesterstheE2F1transcriptionfactor.InteractswithheterodimericE2F/DPtranscriptionfactorcomplexescontainingTFDP1andeitherE2F1,E2F3,E2F4orE2F5,orTFDP2andE2F4.TheunphosphorylatedforminteractswithEID1,ARID3B,KDM5A,SUV39H1,MJD2A/JHDM3AandTHOC1.InteractswiththeN-terminaldomainofTAF1.InteractswithAATF,DNMT1,LIN9,LMNA,SUV420H1,SUV420H2,PELP1andTMPO-alpha.MayinteractwithNDC80.InteractswithGRIP1andUBR4.InteractswithARID4AandKDM5B.InteractswithE4F1andLIMD1.InteractswithSMARCA4/BRG1ANDHDAC1Bysimilarity.InteractswithadenovirusE1Aprotein,HPVE7proteinandSV40largeTantigen.InteractswithPSMA3andUSP4.Interacts(whenmethylatedatLys-860)withL3MBTL1.
      SUBCELLULARLOCATION:Nucleus.
      TISSUESPECIFICITY:Expressedintheretina.
      DOMAIN:ThePocketdomainbindstothethreonine-phosphorylateddomainC,therebypreventinginteractionwithheterodimericE2F/DPtranscriptionfactorcomplexes.
      PTM:PhosphorylatedinG1,therebyreleasingE2F1whichisthenabletoactivatecellgrowth.DephosphorylatedatthelateMphase.SV40largeTantigen,HPVE7andadenovirusE1Abindtotheunderphosphorylated,activeformofpRb.PhosphorylationatThr-821andThr-826promotesinteractionbetweentheC-terminaldomainCandthePocketdomain,andtherebyinhibitsinteractionswithheterodimericE2F/DPtranscriptionfactorcomplexes.DephosphorylatedatSer-795bycalcineruinuponcalciumstimulation.
      N-terminusismethylatedbyMETTL11A/NTM1Bysimilarity.MonomethylatedatLys-860bySMYD2,promotinginteractionwithL3MBTL1.
      INVOLVEMENTINDISEASE:DefectsinRB1arethecauseofchildhoodcancerretinoblastoma(RB)[MIM:180200].RBisacongenitalmalignanttumorthatarisesfromthenuclearlayersoftheretina.Itoccursinabout1:20"000livebirthsandrepresentsabout2%ofchildhoodmalignancies.Itisbilateralinabout30%ofcases.AlthoughmostRBappearsporadically,about20%aretransmittedasanautosomaldominanttraitwithincompletepenetrance.Thediagnosisisusuallymadebeforetheageof2yearswhenstrabismusoragraytoyellowreflexfrompupil("cateye")isinvestigated.
      DefectsinRB1areacauseofsusceptibilitytobladdercancer(BLC)[MIM:109800].Amalignancyoriginatingintissuesoftheurinarybladder.Itoftenpresentswithmultipletumorsappearingatdifferenttimesandatdifferentsitesinthebladder.Mostbladdercancersaretransitionalcellcarcinomas.Theybeginincellsthatnormallymakeuptheinnerliningofthebladder.Othertypesofbladdercancerincludesquamouscellcarcinoma(cancerthatbeginsinthin,flatcells)andadenocarcinoma(cancerthatbeginsincellsthatmakeandreleasemucusandotherfluids).Bladdercancerisacomplexdisorderwithbothgeneticandenvironmentalinfluences.
      DefectsinRB1areacauseofosteogenicsarcoma(OSRC)[MIM:259500].
      SEQUENCESIMILARITIES:Belongstotheretinoblastomaprotein(RB)family.
      MolecularWeight~110observed
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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