Immunogen | Purifiedhuman-fibronectinfromamnionliquid. |
Epitope | cellattachmentfragment |
Clone | 3E3 |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Mouse |
Specificity | Theantibodyreactswithhumanfibronectinandwithapepticfragment(11.5kD)fromhumanfibronectin(Pierschbackeretal.,1981)comprimisingthecellattachmentsite(Pierschbackeretal.,1981;Pierschabackeretal.,1983,1984;Haymanetal.,1985;Ruoslahtietal.,1986;Hynes,1985).Itinhibitstheattachmentofnormalratkidneycellstohumanfironectincoatedplasticsurface(Pierschbackeretal.,1981).BindingoftheantibodytofibronectincannotbeinhibitedbycelladhesionpeptidesfromfibronectinhavingthesequenceArg-Gly-AspofGly-Arg-Gly-Asp-Ser(Pierschabackeretal.,1984;Haymanetal.,1985;Ruoslahtietal.,1986). |
Isotype | IgG1 |
SpeciesReactivity | |
AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Thisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined. |
GeneSymbol | - FN1
- DKFZp686H0342
- DKFZp686I1370
- MSF
- CIG
- LETS
- DKFZp686O13149
- FN
- DKFZp686F10164
- FINC
|
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity). SIZE:2386aminoacids;262607Da SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1. SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix. TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage. PTM:Sulfated. SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains. |