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Millipore/MAB5326 | Anti-Nestin Antibody, clone 10C2/MAB5326/100 µg
  • Millipore/MAB5326 | Anti-Nestin Antibody, clone 10C2/MAB5326/100 µg

Millipore/MAB5326 | Anti-Nestin Antibody, clone 10C2/MAB5326/100 µg

價格: ¥4068.00 市場價: 6780.00

貨號: MAB5326
品牌: Millipore
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    • Description
      CatalogueNumberMAB5326
      ReplacesAB5922
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-NestinAntibody,clone10C2
      BackgroundInformationNestin,alargeintermediatefilamentprotein(classTypeVI)expressedduringdevelopmentandinmyotendinousandneuromuscularjunctions.Nestinexpressionisrestricted,typicallydisappearingbyE18.NestinisthoughttobeareasonableneuronalMarker;however,recentstudieshavefoundnestinexpressioninothercelltypessuchasendothelialcells{FoliaBIOLOGica,44(5):155,1998}.Nestinidentifiesthemostprimitiveneuroepitheliumbutalsoidentifiesmanyotherembryonictissues,soitisnotspecificforCNS.NestinexpressionisseeninalmostallGBMs(Glioblastomamulitformes)andmanymelanomas(bothprimaryandmetastatic)butnotinanymetastaticcarcinoma.
      ProductInformation
      FormatPurified
      Control
      • PositiveControl:WI-38(ATCC#CCL75)orU251celllines
      PresentationProteinAPurifiedmouseimmunoglobulinin20mMsodiumphosphate,250mMNaCl,pH.7.6,with0.1%sodiumazideasapreservative.
      StorageandShippingInformation
      StorageConditionsAntibodiesmaybestoredat4°Cor-20°C.Avoidrepeatedfreeze/thawcycles.

      Duringshipment,smallvolumesofproductwilloccasionallybecomeentrappedinthesealoftheproductvial.Forproductswithvolumesof200μLorless,werecommendgentlytappingthevialonahardsurfaceorbrieflycentrifugingthevialinatabletopcentrifugetodislodgeanyliquidinthecontainer"scap.
      Applications
      ApplicationThisAnti-NestinAntibody,clone10C2isvalidatedforuseinIC,IH,IH(P),WBforthedetectionofNestin.
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      • WesternBlotting
      ApplicationNotesImmunoblotting:1:1,000-1:2,000ondenaturedprotein.Westernblot:1:1000-1:5000.Recognizesa220-240kDAbandondenaturingSDSgels.Thereisalsoa60-64kDabandpresentinmostlysates,theidentityofthissmallerfragmentisunknownbutitisnotseeninlysatesnotcontaininghumannestinprotein.

      WI-38(ATCC#CCL75)canbeusedasapositivecontrol.U251celllineisalsopositive.

      Immunocytochemistry:1:200oncellsfixedin4%paraformaldehydeandpermeABIlizedwith0.02%Triton

      Immunohistochemistry:paraformaldehydeisthepreferredfixative:1:200onformalinfixed,paraffinembeddedtissuesections.Usetriton0.02%inblockonly;dilutionbuffer0.1%BSA;incubations1-2hoursRT.Humanfetalbrainissuggestedasapositivecontrol.(CAMessametal,Develop.BrainRes,134:87-92,2002;Gu,etal.,BrainRes.943:174-180,2002).Filamentousstaininginthecytoplasmofcells

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BiologicalInformation
      ImmunogenFusionprotein.
      Epitopehuman
      Clone10C2
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityReactswithhumanNestin,withweakreactivitytomonkeynestin.Notreactivewithrodentnestin.
      IsotypeIgG1
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryNestinisanintermediatefilamentproteinthatwasfirstidentifiedwithamonoclonalantibodybyHockfieldandMcKay(1985)[PubMed4078630].Itisexpressedpredominantlyinstemcellsofthecentralnervoussystemintheneuraltube.Uponterminalneuraldifferentiation,nestinisdownregulatedandreplacedbyneurofilaments.[suppliedbyOMIM]
      GeneSymbol
      • NES
      • ORFNames=Nbla00170
      • FLJ21841
      • Nestin.
      • Nbla00170
      Non-ReactiveSpecies
      • Mouse
      • Rat
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P48681#MayplayaroleinthetraffickinganddistributionofIFproteinsandpotentiallyothercellularfactorstodaughtercellsduringProgenitorcelldivision(Bysimilarity).
      SIZE:1621aminoacids;177439Da
      SUBUNIT:InteractswithFHOD3(Bysimilarity).
      TISSUESPECIFICITY:CNSstemcells.
      DEVELOPMENTALSTAGE:Uponterminalneuraldifferentiation,nestinisdown-regulatedandreplacedbyneurofilaments.
      SIMILARITY:SwissProt:P48681##Belongstotheintermediatefilamentfamily.
      StemCellType
      • NeuralStemCells
      • EpithelialCells
      MolecularWeight200-220kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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