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Millipore/MAB5300 | Anti-Dopamine Antibody, clone K56A/MAB5300/100 µL
  • Millipore/MAB5300 | Anti-Dopamine Antibody, clone K56A/MAB5300/100 µL

Millipore/MAB5300 | Anti-Dopamine Antibody, clone K56A/MAB5300/100 µL

價格: ¥4308.00 市場價: 7180.00

貨號: MAB5300
品牌: Millipore
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    • Description
      CatalogueNumberMAB5300
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-DopamineAntibody,cloneK56A
      ProductInformation
      FormatPurified
      PresentationPurifiedimmunoglobulin.Liquidcontaining50%glycerol.
      StorageandShippingInformation
      StorageConditionsMaintainat-20°Cinundilutedaliquotsforupto6monthsafterdateofreceipt.
      Applications
      ApplicationAnti-DopamineAntibody,cloneK56AdetectslevelofDopamine&hasbeenpublished&validatedforuseinIH.
      KeyApplications
      • Immunohistochemistry
      ApplicationNotesImmunohistochemistry:1:500-1:2,500usingfreefloatingsectionsbythePAPtechniqueonratdopaminergicareas.

      Optimalworkingdilutionsmustbedeterminedbyenduser.

      PROTOCOLforDopamineDetectionbyImmunohisto/cytochemistry.Exampleforaratbrain.

      1.SOLUTIONSTOBEPREPARED-Solutionmustbepreparedasneeded.

      SolutionA:0.1Mcacodylate,10g/LsodiummetABIsulfite,pH6.2.

      SolutionB:0.1Mcacodylate,10g/Lsodiummetabisulfite,3-5%glutaraldehyde,pH7.5.

      2.RATPERFUSION-TheratisanaesthetizedwithsodiumpentobarbitalorNembutalandperfusedintracardiallythroughtheaortausingapumpwithSolutionA(30mL):150-300mL/min,SolutionB(500mL):150-300mL/min.

      3.POSTFIXATION:15to30minutesinSolutionB,then4softwashesin0.05MTriswith8.5g/Lsodiummetabisulfite,pH7.5(SolutionC).

      4.TISSUESECTIONING:Vibratomeorcryostatsectionscanbeused.

      5.REDUCTIONSTEP:SectionsarereducedwithSolutionCcontaining0.1Msodiumborohydridefor10minutes.Thesectionsarewashed4timesinsolutionCwithoutsodiumborohydride.

      6.APPLICATIONOFDOPAMINEANTIBODY:Useafinaldilutionof1:2,500-1:10,000inSolutionCcontaining0.1%TritonX100and2%non-specificserum.Incubate12sectionsper2mLdilutedantibodyovernight,+4°C.Thenwashthesectionsthreetimesfor10minuteseachinSolutionC.(Notethattheantibodymaybeusableatahigherdilution.Thisshouldbeexploredtominimizethepossibilityofhighbackground.Additionally,notethatachangeinthebufferingsystemasindicatedintheprotocolmaychangethebackgroundandantibodyrecognition).ThespecificreactionisthenrevealedbyPAPprocedure.

      6.SECONDANTIBODY:Incubatethesectionswitha1:50to1:200dilutionofgoatanti-rabbitinSolutionBcontaining1%non-specificserumforeither3hrsat20°Cor2hrat37°C.Thenwashthesections,3times,for10minuteseachwithSolutionB.

      7.PAP:Incubatethesectionswiththeappropriatedilutionofperoxidaseanti-peroxidase(forfreefloatingmethod)inSolutionBcontaining1%non-specificserumfor1-2hoursat37°C.Thenwashsections3timesfor10mineachinsolutionB.

      8.VISUALIZATION:Theantigen-antibodycomplexesarevisualizedusingDAB-4-HCl(25mg/100mL)(orotherchromogen)in0.05MTrisandfiltrated;0.05%hydrogenperoxideisadded.Incubatethesectionsfor10minutesatroomtemp.Stopthereactionbytransferringthesectionsto5mL0.05MTris.WashtissuewithsolutionDusing2,10minwashes.Mountsectionsonchrome-alumcoatedslides.Dryovernightat37°C.Rehydratesectionsusingconventionalhistologicalprocedures.Coverslipusingrapidmountingmedia.

      Forresearchuseonly;notforuseasadiagnostic.
      BIOLOGicalInformation
      ImmunogenDopamine-Gluteraldehyde-BSA.
      CloneK56A
      HostMouse
      SpecificityDopamine.

      Thecross-reactivitiesweredeterminedusinganELISAtestbycompetitionexperimentswiththefollowingcompounds:

      CompoundCross-reactivity

      Dopamine-G-BSA1

      L-DOPA-G-BSA1/10,000

      Tyrosine-G-BSA1/36,000

      Tyramine-G-BSA1/>50,000

      Noradrenaline-G-BSA1/>50,000

      Octopamine-G-BSA1/>50,000

      Adrenaline-G-BSA1/>50,000

      Dopamine1/>50,000

      Abbreviations:

      (G)Glutaraldehyde

      (BSA)BovineSerumAlbumin
      IsotypeIgG
      SpeciesReactivity
      • Rat
      AntibodyTypeMonoclonalAntibody
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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