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Millipore/MAB5292 | Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B/MAB5292/100 µg
  • Millipore/MAB5292 | Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B/MAB5292/100 µg

Millipore/MAB5292 | Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B/MAB5292/100 µg

價格: ¥3948.00 市場價: 6580.00

貨號: MAB5292
品牌: Millipore
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    • Description
      CatalogueNumberMAB5292
      ReplacesAB1791P
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-DopamineD1BReceptorAntibody,cloneSG4-D1B
      AlternateNames
      • DopamineD5Receptor
      ProductInformation
      FormatPurified
      PresentationPurifiedimmunoglobulin.Liquidin0.02MPhosphatebuffer,0.25MNaCl,pH7.6with0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainat2-8°Cinundilutedaliquotsforupto6monthsafterdateofreceipt.
      Applications
      ApplicationThisAnti-DopamineD1BReceptorAntibody,cloneSG4-D1BisvalidatedforuseinIH,WBforthedetectionofDopamineD1BReceptor.
      KeyApplications
      • Immunohistochemistry
      • WesternBlotting
      ApplicationNotesWesternblot:1:250-1:500onmousebrainlysate(unboiledsample)

      Immunohistochemistry

      Optimalworkingdilutionsmustbedeterminedbytheenduser.

      SUGGESTEDWESTERNBLOTPROTOCOL

      Solutions

      10XTransferbuffer1liter

      Tris115.3gm

      Glycine24.2gm

      [SDS4gm(maybeomitted)]

      Fora1Xworkingsolution:1liter

      mix700mLwater

      200mL

      MeOH100mL

      10XTransfer

      TBSTstock1liter1M

      TrispH7.510mL

      5MNaCl30mL

      10%Tween-205Ml

      1.RunSDS-PAGEgelasdesired.

      2.SoakPVDFmembrane(ImmobilonPfromMillipore)in100%methanolfor1-2minutestowetthemembrane.

      3.Soakmembraneintransferbuffer(seebelow)untilreadytosetupblot(>15min)transfer.

      4.Assemblegel-transfersandwichwiththeblottowardthepositiveelectrode.

      5.Transferproteinfromgeltomembraneat100voltsfor1-2hoursforsmallmolecularweightproteinsor65mAfor16hours(overnight)forcompletetransferofhighermolecularweightproteins.

      6.StainthetransferredbandswithChemiconBLOT-FastStain(CatalogNumber2076).

      7.Destainwithdeionizedwater.

      8.Blockwith5%non-fatmilk(MarvelorCarnation)inwater,overnightat4°C.Thenon-fatmilkshouldbedissolvedfreshly,centrifuged10,000rpmfor10min,andfilteredthroughglassfilter(GelmanAcrodisc).

      9.Incubationwithfirstantibodyovernightwithrockingat4°Cinblockingsolution.Optimalworkingdilutionsandincubationtimewillneedtobedeterminedbytheenduser.

      10.Washatleast3x5min.withTBST.Fromthisstage,azideshouldbeomitted.

      11.Incubationwiththesecondaryantibody(HRP-conjugatedgoatanti-mouseantibody,forexampleChemiconCatalogNumberAP124P,dilutedappropriately)1hatroomtemperature.

      12.Washatleast3x5min.withTBST.

      13.PerformECLwithcommercialkit(femtoLUCENT,ChemiconCatalogNumber2078).
      BIOLOGicalInformation
      ImmunogenRecombinantratD1bReceptor,carboxyterminus.
      CloneSG4-D1B
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityDopamineReceptorD1b(D5).
      IsotypeIgG1
      SpeciesReactivity
      • Mouse
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodestheD5subtypeofthedopaminereceptor.TheD5subtypeisaG-proteincoupledreceptorwhichstimulatesadenylylcyclase.Thisreceptorisexpressedinneuronsinthelimbicregionsofthebrain.Ithasa10-foldhigheraffinityfordopaminethantheD1subtype.Pseudogenesrelatedtothisgeneresideonchromosomes1and2.
      GeneSymbol
      • DRD5
      • DRD1B
      • DRD1L2
      • DBDR
      • D
      • MGC10601
      Non-ReactiveSpecies
      • Human
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P21918#Thisisoneofthefivetypes(D1toD5)ofreceptorsfordopamine.TheactivityofthisreceptorismediatedbyGproteinswhichactivateadenylylcyclase.
      SIZE:477aminoacids;52951Da
      SUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein.
      TISSUESPECIFICITY:Neuron-specific,localizedprimarilywithinlimbicregionsofthebrain.
      DISEASE:SwissProt:P21918#DefectsinDRD5maybeacauseofschizophrenia,butnoproofhasyetbeenfound.&DefectsinDRD5areacauseofblepharospasm[MIM:606798].Itisaprimaryfocaldystoniaaffectingtheorbicularisoculimuscles,usuallybeginninginmiddleage.Initialsymptomsincludeeyeirritationandfrequentblinking,progressingtoinvoluntaryspasmsofeyelidclosure.Inseverecases,thiscanleadtofunctionalblindness.
      SIMILARITY:SwissProt:P21918##BelongstotheG-proteincoupledreceptor1family.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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