Immunogen | Ratbrainmicrotubule-associatedprotein. |
Clone | AA6 |
Host | Mouse |
Specificity | ReactsspecificallywithMAP1B(MAP5)byimmunoblot.Showsselectivestainingofdendritictreesthroughoutthebrain.Nocross-reactivityobservedtootherMAPsortubulin.
MonoclonalAnti-MAP1bisimmunospecificforMAP1b(alsoknownasMAP5,MAPIXorMAP1.2;Riedereretal.,1986;Tuckeretal.,1988)asdeterminedbyanimmunoblotassay.Additionofantibodytomicrotubuleproteinsbeforepolymerizationresultsinabnormallyshort(butotherwisemorphologicallynormal)microtubules. |
Isotype | IgG1 |
SpeciesReactivity | - Bovine
- Chicken
- Hamster
- Mouse
- Rat
- Feline
|
AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Thisgeneencodesaproteinthatbelongstothemicrotubule-associatedproteinfamily.Theproteinsofthisfamilyarethoughttobeinvolvedinmicrotubuleassembly,whichisanessentialstepinneurogenesis.TheproductofthisgeneisaprecursorpolypeptidethatpresumablyundergoesproteolyticprocessingtogeneratethefinalMAP1BheavychainandLC1lightchain.Geneknockoutstudiesofthemousemicrotubule-associatedprotein1Bgenesuggestedanimportantroleindevelopmentandfunctionofthenervoussystem. |
GeneSymbol | - MAP1B
- DKFZp686F1345
- FUTSCH
- DKFZp686E1099
- MAP5
- FLJ38954
|
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P46821#ThefunctionofbrainMAPSisessentiallyunknown.PhosphorylatedMAP1Bmayplayaroleinthecytoskeletalchangesthataccompanyneuriteextension.PossIBLyMAP1BBindstoatleasttwotubulinsubunitsinthepolymer,andthisbridgingofsubunitsmightbeinvolvedinnucleatingmicrotubulepolymerizationandinstABIlizingmicrotubules. SIZE:2468aminoacids;270620Da SUBUNIT:3differentlightchains,LC1,LC2andLC3,canassociatewithMAP1AandMAP1Bproteins.LC1interactswiththeamino-terminalregionofMAP1B.InteractswithANP32A(Bysimilarity). DOMAIN:SwissProt:P46821HasahighlybasicregionwithmanycopiesofthesequenceKKEEandKKEI/V,repeatedbutnotatfixedintervals,whichisresponsibleforthebindingofMAP1Btomicrotubules. PTM:LC1isgeneratedfromMAP1Bbyproteolyticprocessing.&PhosphorylateduponDNAdamage,probablybyATMorATR. SIMILARITY:BelongstotheMAP1family. |