Mouse monoclonal antibody raised against native ACR.
Immunogen:
Native purified porcine ACR.
Host:
Mouse
Theoretical MW (kDa):
55, 53, 45, 35
Reactivity:
Pig
Specificity:
This antibody reacts with various forms of porcine acrosin (55, 53, 45 and 35 KDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
Form:
Liquid
Isotype:
IgG1
Recommend Usage:
The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction:
Store at 4°C. Do not freeze.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro.Peknicova J, Capkova J, Geussova G, Ivanova M, Mollova M.Theriogenology. 2001 Jul 15;56(2):211-23.
2.
Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction.Moos J, Peknicova J, Tesarik J.Biol Reprod. 1993 Aug;49(2):408-15.
3.
Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction.Moos J, Peknicova J, Tesarik J.Biochim Biophys Acta. 1993 Apr 16;1176(3):199-207.
4.
Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin.Moos J, Tesarik J, Leca G, Peknicova J.FEBS Lett. 1991 Dec 2;294(1-2):27-30.
5.
Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test.Peknicova J, Moos J.Andrologia. 1990 Sep-Oct;22(5):427-35.
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