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Millipore/MAB3418X | Anti-MAP2 Antibody, Alexa Fluor? 488 conjugated/MAB3418X/100 µg
  • Millipore/MAB3418X | Anti-MAP2 Antibody, Alexa Fluor? 488 conjugated/MAB3418X/100 µg

Millipore/MAB3418X | Anti-MAP2 Antibody, Alexa Fluor? 488 conjugated/MAB3418X/100 µg

價格: ¥5052.00 市場價: 8420.00

貨號: MAB3418X
品牌: Millipore
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    • Description
      CatalogueNumberMAB3418X
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-MAP2Antibody,AlexaFluor?488conjugated
      ProductInformation
      FormatAlexaFluor?488
      Control
      • Brain,Neuronalculture,HumanglioblastomaT98Gcells
      PresentationPurifiedimmunoglobulinconjugatedtoAlexaFluor?488.LiquidinPhosphatebufferwith15mg/mLBSAasastABIlizerand0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainfor1yearat2–8°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationThisAnti-MAP2Antibody,AlexaFluor488conjugatedisvalidatedforuseinIHforthedetectionofMAP2.
      KeyApplications
      • Immunohistochemistry
      ApplicationNotesImmunohistochemistry

      Optimalworkingdilutionsmustbedeterminedbyenduser.

      APPLICATIONNOTES:

      Immunohistochemistry:Anti-MAP-2canbeusedtostaintissue(brainorspinalcord)fixedwithparaformaldehyde.
      BIOLOGicalInformation
      ImmunogenBovinebrainmicrotubuleprotein.
      CloneAP20
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityRecognizesMAP-2(microtubuleassociatedprotein-2).MAP-2isoneofseveralhighmolecularweightproteinsthatplayanimportantroleinbrainmicrotubuleassembly.Inadditiontoitsassociationwithmicrotubules,MAP-2associateswithneurofilamentsandactinfilamentssuggestingthatitmayguideinteractionamongmicrotubules,othercytoskeletalelements,andcytoplasmicorganelles(1).

      MAP-2isastringentMarkerforneurons.Inaddition,MAP-2displaysintracellularspecificity.Inthecentralnervoussystem,MAP-2isconfinedtoneuronalcellbodiesanddendrites.Thereareexceptions,however,wheresomeaxonsstainpositiveforsmallamountsofMAP-2(2,3).MAP-2isuniformlydistributedthroughoutthecellwhenfirstexpressedinculturedneuronsbutbecomesselectivelylocalizedasdendriticdevelopmentproceeds(4,5).

      SDS-PAGEProfiles:InSDS-PAGEMAP-2fromadultratmigratesasacloselyassociateddoublethavingamolecularweightofapproximately300kD.However,earlyinbraindevelopment(postnatalday10inrats),MAP-2migratesasasinglebandthatisidenticaltothelowermolecularweightbandoftheadultMAP-2doublet(MAP-2b).Laterindevelopment(postnataldays17-18),themobilityofMAP-2changestotheadultdoubletform.(Inthespinalcord,conversiontotheadultformoccursearlier).
      IsotypeIgG1
      SpeciesReactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesaproteinthatbelongstothemicrotubule-associatedproteinfamily.Theproteinsofthisfamilyarethoughttobeinvolvedinmicrotubuleassembly,whichisanessentialstepinneurogenesis.Theproductsofsimilargenesinratandmouseareneuron-specificcytoskeletalproteinsthatareenrichedindentrites,implicatingaroleindeterminingandstabilizingdentriticshapeduringneurondevelopment.Anumberofalternativelysplicedvariantsencodingdistinctisformshavebeendescribed.
      GeneSymbol
      • MAP2
      • MAP2B
      • MAP-2
      • MAP2C
      • DKFZp686I2148
      • MAP2A
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P11137#TheexactfunctionofMAP2isunknownbutMAPsmaystabilizethemicrotubulesagainstdepolymerization.Theyalsoseemtohaveastiffeningeffectonmicrotubules.
      SIZE:1827aminoacids;199539Da
      PTM:MAP2A/cisphosphorylated.PhosphorylateduponDNAdamage,probablybyATMorATR.
      SIMILARITY:SwissProt:P11137##Contains3Tau/MAPrepeats.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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