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Millipore/MAB3400 | Anti-Vimentin Antibody, clone V9/MAB3400/40 µg
  • Millipore/MAB3400 | Anti-Vimentin Antibody, clone V9/MAB3400/40 µg

Millipore/MAB3400 | Anti-Vimentin Antibody, clone V9/MAB3400/40 µg

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貨號(hào): MAB3400
品牌: Millipore
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    • Description
      CatalogueNumberMAB3400
      ReplacesMAB3410
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-VimentinAntibody,cloneV9
      BackgroundInformationVimentinisthemajorsubunitproteinoftheintermediatefilamentsofmesenchymalcells.Itisbelievedtobeinvolvedwiththeintracellulartransportofproteinsbetweenthenucleusandplasmamembrane.Vimentinhasbeenimplicatedtobeinvolvedintherateofsteroidsynthesisviaitsroleasastoragenetworkforsteroidogeniccholesterolcontaininglipiddroplets.ImmunohistochemicalstainingforVimentinischaracteristicofsarcomas(ofneural,muscleandfibroblastorigin)comparedtocarcinomaswhicharegenerallynegative.Melanomas,lymphomasandvasculartumorsmayallstainforVimentin.
      ProductInformation
      FormatPurified
      Control
      • Breastcarcinomatissue,Colon,Prostate,Tonsil,Tonsilarlymphoma,Uterus,Ureter
      PresentationPurifiedmousemonoclonalIgG1presentedin0.02MPhosphatebuffer,0.25MNaClwith0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat2-8oCfromdateofreceipt.
      Applications
      ApplicationAnti-VimentinAntibody,cloneV9isanantibodyagainstVimentinforuseinIC,IH,IH(P)&WB.
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      • WesternBlotting
      ApplicationNotesImmunoblotting:
      Adilutionof1:1000wasusedinapreviouslot.

      Immunohistochemistry:
      5-10μg/mLwasusedonapreviouslot;canbeusedonformalinfixedparaffinembeddedtissueswithtrADItionalhightemperature,citratebuffertreatments.Prolongedformalinfixationcanreduceantivbodyreactivity.

      Immunocytochemistry:
      5-10μg/mLofapreviouslotwasusedinIC.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenPurifiedvimentinfrompigeyelens
      CloneV9
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityRecognizesa57-60kDaprotein.ShowsnocrossreactivitywithothercloselyrelatedintermediatefilamentproteinsincludingdesminandGFAP.Intissuesections,thisreagentshowspositivereactiononconnectivetissue,endothelialcells,vascularsmoothmusclecellsandastrocytes.Intissueculture,typicalintermediatefilamentprofilesareseeninfibroblastsandothercelltypes,whichcontainvimentin
      IsotypeIgG1
      SpeciesReactivity
      • Bovine
      • Canine
      • Chicken
      • Feline
      • Human
      • Monkey
      • Pig
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryAlongwiththeMICROFILaments(actins)andmicrotubules(tubulins),theintermediatefilamentsrepresentathirdclassofwell-characterizedcytoskeletalelements.Thesubunitsdisplayatissue-specificpatternofexpression.Desmin(MIM125660)isthesubunitspecificformuscleandvimentinthesubunitspecificformesenchymaltissue.[suppliedbyOMIM]
      GeneSymbol
      • VIM
      • FLJ36605
      Non-ReactiveSpecies
      • Mouse
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P08670#Vimentinsareclass-IIIintermediatefilamentsfoundinvariousnon-epithelialcells,especiallymesenchymalcells.
      SIZE:466aminoacids;53652Da
      SUBUNIT:Homopolymer.ComponentofacomplexcomposedatleastofACTB,AP2M1,AP2A1,AP2A2,MEGF10andVIM.InteractswithHCVcoreprotein.
      TISSUESPECIFICITY:Highlyexpressedinfibroblasts,someexpressioninT-andB-lymphocytes,andlittleornoexpressioninBurkitt"slymphomacelllines.Expressedinmanyhormone-independentmammarycarcinomacelllines.
      PTM:Oneofthemostprominentphosphoproteinsinvariouscellsofmesenchymalorigin.Phosphorylationisenhancedduringcelldivision,atwhichtimevimentinfilamentsaresignificantlyreorganized.
      SIMILARITY:SwissProt:P08670##Belongstotheintermediatefilamentfamily....
      MolecularWeight57-60kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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