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Millipore/MAB2081 | Anti-β-Catenin Antibody, clone 5H10/MAB2081/100 µg
  • Millipore/MAB2081 | Anti-β-Catenin Antibody, clone 5H10/MAB2081/100 µg

Millipore/MAB2081 | Anti-β-Catenin Antibody, clone 5H10/MAB2081/100 µg

價格: ￥3576.00 市場價: 5960.00
貨號: MAB2081
品牌: Millipore
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    • Description
      CatalogueNumberMAB2081
      Replaces04-1002
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-β-CateninAntibody,clone5H10
      BackgroundInformationThetransmembrane,calciumdependentmoleculeE-cadherinisconsideredtobeoneofthekeymoleculesintheformationoftheintercellularjunctionalcomplexandestablishmentofpolarityinepithelialcells.Catenins,thealpha-subunit(102kDa),beta-subunit(88kDa)andgamma-subunit(82kDa),[reviewedinTakeichi,1991]areagroupofproteinsthatinteractwiththeintercellulardomainofE-cadherin,resultingincomplexesofE-cadherin/beta-catenin/alpha-cateninorE-cadherin/gamma-catenin/alpha-Catenin(Hincketal.,1994).Beta-catenin,thevertebratehomologueofDrosophilasegmentpolaritygenearmADIllo,andkeyelementoftheWntsignaltransductionpathways,isimportantinembryogenesis,celltransformation,andintercellularadhesionincludingtheadhesion,motilityandmetastasisofcancercells.Thus,cateninproteinexpression,regulationandlocalizationarepotentiallyimportantMarkerstopredictmotilityandinvasivenessofepithelialneoplasms.Theregionsofbothalpha-andbeta-catenin,locatedon5q21-22and3p21,havebeenshowntobeinvolvedinthedevelopmentofcertaintumors(seeJiang,1996;Schlosshaueretal.,2002)andreducedexpressionsofbothalpha-andbeta-cateninhavebeendescribedinvarioustumorsincludingbreastcarcinoma(Hashizumeetal,1996).Besidesadhesionfunctions,beta-cateninbindstothetumorsuppressorgeneAPC.APCmutationdisturbstheequilibriumandcytoplasmiclevelsoffreebeta-cateninlevelinthecellandmayhavearoleintumOrigenesis.
      ProductInformation
      FormatPurified
      Control
      • A431andHT1080celllineextracts
      PresentationProteinAPurifiedIgGinliquidat1mg/mLin0.02MPBwith0.25MNaCl,pH7.6,containing0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainfor1yearat2–8°Cfromdateofshipment.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationAnti-β-CateninAntibody,clone5H10isaMouseMonoclonalAntibodyfordetectionofβ-Cateninalsoknownasbetacatenin&hasbeentestedinICC,IHC,IHC(P),IP&WB.
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      • Immunoprecipitation
      • WesternBlotting
      ApplicationNotesImmunohistochemistry:Lowcross-linkingfixativesarerecommended.Histochoicefixativeiseffective.FormalinandPFAareeffective,howevermayshowweakerstaining.

      Immunohistochemistryonformalinfixedparaffinembeddedtissuesrequireslightfixationsandcitricacid/microwaveantigenrecoveryforstaining;Inbrief,sectionsof2μmthicknessweretreatedafterdeparaffinizationwith0.3%hydrogenperoxideinmethanolfor30minutestoinhibitendogenousperoxidaseandwashedwithTBSsolution(Tris-bufferedsalinepH7.6).Toenhanceantigenretrieval,sectionsweremicrowave-pretreatedin0.01Mcitratebuffersolutionat750Wfora3minute-cycleperiodrepeatedthreetimes;amplifieddetectionmethodsarerecommended.

      Immunocytochemistry:EffectiveforA431andHT1080celllineswithacetoneormethanol,orlightPFAfixation.

      Immunoblotting

      Immunoprecipitation

      Optimalworkingdilutionsmustbedeterminedbyend-user.
      BIOLOGicalInformation
      ImmunogenProducedbyimmunizationwithrecombinanthumanbeta-catenin.
      Clone5H10
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityRecognizesanepitopeinthecarboxyterminus,aminoacids758-771,ofhumanbeta-catenin.
      IsotypeIgG1
      SpeciesReactivity
      • Human
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryBeta-cateninisanadherensjunctionprotein.Adherensjunctions(AJs;alsocalledthezonulaadherens)arecriticalfortheestablishmentandmaintenanceofepitheliallayers,suchasthoseliningorgansurfaces.AJsmediateadhesionbetweencells,communicateasignalthatneighboringcellsarepresent,andanchortheactinCytoskeleton.Inservingtheseroles,AJsregulatenormalcellgrowthandbehavior.Atseveralstagesofembryogenesis,woundhealing,andtumorcellmetastasis,cellsformandleaveepithelia.Thisprocess,whichinvolvesthedisruptionandreestablishmentofepithelialcell-cellcontacts,mayberegulatedbythedisassemblyandassemblyofAJs.AJsmayalsofunctioninthetransmissionofthe"contactinhibition"signal,whichinstructscellstostopdividingonceanepithelialsheetiscomplete.[suppliedbyOMIM]
      GeneSymbol
      • CTNNB1
      • FLJ25606
      • CTNNB
      • Beta-catenin
      • PRO2286
      • FLJ37923
      • DKFZp686D02253
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P35222#InvolvedintheregulationofcelladhesionandinsignaltransductionthroughtheWntpathway.
      SIZE:781aminoacids;85497Da
      SUBUNIT:Twoseparatepoolsarefoundinthecytoplasm:oneisPSEN1/cadherin/catenincomplexwhichanchorstotheactincytoskeleton.TheotherpoolispartofalargecomplexcontainingAXIN1,AXIN2,APC,CSNK1A1andGSK3BthatpromotesphosphorylationonN-terminalSerandThrresiduesandubiquitinationofCTNNB1viaBTRCanditssubsequentdegradationbytheproteasome.Wnt-dependentactivationofDVLantagonizestheactionofGSK3B.WhenGSK3Bactivityisinhibitedthecomplexdissociates,CTNNB1isdephosphorylatedandisnolongertargetedfordestruction.ThestABIlizedproteintranslocatestothenucleus,whereitbindsTCF/LEF-1familymembers,TBP,BCL9andpossIBLyalsoRUVBL1andDUPLIN.BindsCTNNBIPandEP300.CTNNB1formsaternarycomplexwithLEF1andEP300thatisdisruptedbyCTNNBIP1binding(Bysimilarity).InteractswiththePDZdomainofTAX1BP3;thisinteractioninhibitsthetranscriptionalactivityofCTNNB1(Bysimilarity).InteractswithAJAP1,BAIAP1,CARM1,CTNNA3,CXADRandPCDH11Y.BindsSLC9A3R1.InteractswithGLIS2.InteractswithSLC30A9(Bysimilarity).
      SUBCELLULARLOCATION:Cytoplasm.Nucleus.Note=Cytoplasmicwhenitisunstabilized(highlevelofphosphorylation)orboundtoCDH1.Translocatestothenucleuswhenitisstabilized(lowlevelofphosphorylation).InteractionwithGLIS2promotesnucleartranslocation.
      TISSUESPECIFICITY:Expressedinseveralhairfolliclecelltypes:basalandperipheralmatrixcells,andcellsoftheouterandinnerrootsheats.Expressedincolon.
      PTM:PhosphorylationbyGSK3BrequirespriorphosphorylationofSer-45byanotherkinase.PhosphorylationproceedsthenfromThr-41toSer-37andSer-33.&EGFstimulatestyrosinephosphorylation.PhosphorylationonTyr-654decreasesCDH1bindingandenhancesTBPbinding.&UbiquitinatedbyaE3ubiquitinligasecomplexcontainingUBE2D1,SIAH1,CACYBP/SIP,SKP1A,APCandTBL1X(Probable).Itsubiquitinationleadstoitssubsequentproteasomaldegradation.
      DISEASE:"SwissProt:P35222#ActivatingmutationsinCTNNB1haveoncogenicactivityresultingintumordevelopment.Somaticmutationsarefoundinvarioustumortypes,includingcoloncancers,ovarianandprostatecarcinomas,hepatoblastoma(HB),hepatocellularcarcinoma(HCC).HBsaremalignantembryonaltumorsmainlyaffectingyoungchildreninthefirstthreeyearsoflife.&DefectsinCTNNB1areacauseofpilomatrixoma(PTR)[MIM:132600];acommonbenignskintumor.&DefectsinCTNNB1areacauseofmedulloblastoma(MDB)[MIM:155255].MDBisamalignant,invasiveembryonaltumorofthecerebellumwithapreferentialmanifestationinchildren.&AchromosomalrearrangementinvolvingCTNNB1maybeacauseofsalivaryglandpleiomorphicadenomas(PA)[181030].Pleiomorphicadenomasarethemostcommonbenignepithelialtumorsofthesalivarygland.Translocationt(3;8)(p21;q12)withPLAG1."
      SIMILARITY:SwissProt:P35222##Belongstothebeta-cateninfamily.&Contains12ARMrepeats.
      MolecularWeight94kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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