Immunogen | ChondroitinaseABC-digestedadulthumanaggrecan. |
Clone | MK-302 |
Host | Mouse |
Specificity | Reactswithawiderangeofcartilageproteoglycansaftereitherchondroitinase(ABCorAC)ortesticularhyaluronidasedigestion.NativeproteoglycansdonotreactwithMAB2035.Antibodybindingtoepitopeissuccessfullyinhibitedbydisaccharidesofchondroitin-6-sulfate.Recognizesthechondroitin-6-sulfatestubs(preferentiallyinclusters)ofhighdensitycartilageproteoglycansofdifferentspecies.Nocross-reactivitywithdermatansulfate,keratansulfateorchondroitin-4-sulfate. |
Isotype | IgG1 |
SpeciesReactivity | - Bovine
- Canine
- Human
- Mouse
- Rat
- Rabbit
- Sheep
|
AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Thisgeneisamemberoftheaggrecan/versicanproteoglycanfamily.Theencodedproteinisanintegralpartoftheextracellularmatrixincartilagenoustissueanditwithstandscompressionincartilage.Mutationsinthisgenemaybeinvolvedinskeletaldysplasiaandspinaldegeneration.Multiplealternativelysplicedtranscriptvariantsthatencodedifferentproteinisoformshavebeenobservedinthisgene. |
GeneSymbol | - ACAN
- MSK16
- SEDK
- CSPCP
- AGCAN
- AGC1
- CSPG1
- CSPGCP
|
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P16112#Thisproteoglycanisamajorcomponentofextracellularmatrixofcartilagenoustissues.Amajorfunctionofthisproteinistoresistcompressionincartilage.ItbindsavidlytohyaluronicacidviaanN-terminalglobularregion. SIZE:2415aminoacids;250193Da SUBUNIT:InteractswithFBLN1(Bysimilarity). SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix(Bysimilarity). TISSUESPECIFICITY:Restrictedtocartilages. DEVELOPMENTALSTAGE:Expressionwasdetectedinchondrocytesthroughoutthedevelopingskeleton. DOMAIN:SwissProt:P16112Twoglobulardomains,G1andG2,comprisetheN-terminusoftheproteoglycan,whileanotherglobularregion,G3,makesuptheC-terminus.G1containsLinkdomainsandthusconsistsofthreedisulfide-bondedloopstructuresdesignatedastheA,B,B"motifs.G2issimilartoG1.Thekeratansulfate(KS)andthechondroitinsulfate(CS)attachmentdomainsliebetweenG2andG3. PTM:Containsmostlychondroitinsulfate,butalsokeratansulfatechains,N-linkedandO-linkedoligosaccharides.Thereleaseofaggrecanfragmentsfromarticularcartilageintothesynovialfluidatallstagesofhumanosteoarthritisistheresultofcleavagebyaggrecanase. DISEASE:SwissProt:P16112#DefectsinAGC1arethecauseofspondyloepiphysealdysplasiatypeKimberley(SEDK)[MIM:608361].Spondyloepiphysealdysplasiasareaheterogeneousgroupofcongenitalchondrodysplasiasthatspecificallyaffectepiphysesandvertebrae.TheautosomaldominantSEDKisassociatedwithprematuredegenerativearthropathy. SIMILARITY:Belongstotheaggrecan/versicanproteoglycanfamily.&Contains1C-typelectindomain.&Contains1EGF-likedomain.&Contains1Ig-likeV-type(immunoglobulin-like)domain.&Contains4Linkdomains.&Contains1Sushi(CCP/SCR)domain. |