當(dāng)前位置 ： Abnova >>> Abnova/AKT1 (phospho S473) monoclonal antibody, clone 17F6.B11/1kit/MAB1919
Abnova/AKT1 (phospho S473) monoclonal antibody, clone 17F6.B11/1kit/MAB1919
  • Abnova/AKT1 (phospho S473) monoclonal antibody, clone 17F6.B11/1kit/MAB1919

Abnova/AKT1 (phospho S473) monoclonal antibody, clone 17F6.B11/1kit/MAB1919

價(jià)格: 試用 市場價(jià): 0.00
貨號(hào): MAB1919
品牌: Abnova
規(guī)格
數(shù)量
庫存(0)
特別 提示
代購產(chǎn)品：無質(zhì)量問題不接受退換貨，下單前請仔細(xì)核對信息。
下單后請及時(shí)聯(lián)系客服核對商品價(jià)格，訂單生效后再付款。
資深產(chǎn)品顧問
咨詢顧問

全國免費(fèi)服務(wù)熱線

4000-520-616

  • 自營商城 一站式服務(wù)
  • 廠家直采 剔除溢價(jià)
  • 品質(zhì)甄選 正品保證
  • 嚴(yán)控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說明
  • 常見問題
      • Specification
      • Gene Info
      • Interactome
      • Pathway
      • Disease
      • Related Products
      • List All
      • Specification
      • Product Description:
      • Mouse monoclonal antibody raised against synthetic phosphopeptide of AKT1.
      • Immunogen:
      • Synthetic phosphopeptide corresponding to residues surrounding S473 of human AKT1.
      • Host:
      • Mouse
      • Reactivity:
      • Chimpanzee, Human, Mouse, Rat
      • Specificity:
      • This antibody is specific to human and mouse AKT protein phosphorylated at S473.
      • Form:
      • Liquid
      • Isotype:
      • IgG1, kappa
      • Quality Control Testing:
      • Antibody Reactive Against Synthetic Peptide.
      • Recommend Usage:
      • ELISA (1:20000)Western Blot (1:500-1:3000)Immunohistochemistry (20 ug/mL)The optimal working dilution should be determined by the end user.
      • Storage Buffer:
      • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
      • Storage Instruction:
      • Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
      • Note:
      • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
      • Datasheet:
      • PDF DownloadDownload
      • Publication Reference
      • 1.
      • Phaseolus vulgaris Exerts an Inhibitory Effect on Platelet Aggregation through AKT Dependent Way.Rodríguez-Azúa R, Quinteros EF, Olate-Briones A, Moore-Carrasco R.Prev Nutr Food Sci. 2018 Jun;23(2):102-107. doi: 10.3746/pnf.2018.23.2.102. Epub 2018 Jun 30.
      • 2.
      • PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?Lawlor MA, Alessi DR.J Cell Sci. 2001 Aug;114(Pt 16):2903-10.
      • 3.
      • Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture.Alessi DR.Biochem Soc Trans. 2001 May;29(Pt 2):1-14.
      • 4.
      • Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily.Jones PF, Jakubowicz T, Pitossi FJ, Maurer F, Hemmings BA.Proc Natl Acad Sci U S A. 1991 May 15;88(10):4171-5.
      • Applications
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • Western blot using AKT1 (phospho S473) monoclonal antibody, clone 17F6.B11 (Cat # MAB1919) shows detection of phospho-AKT1 (indicated by arrowhead at ~56 KDa) on PDGF stimulated NIH/3T3 cell lysates (Lane 2).No reactivity is seen for non-phosphorylated AKT1 in untreated cells (Lane 1).Each lane contained approximately 10 ug of lysate.All samples were loaded on to a 4-20% gradient gel for separation.After electrophoresis, the gel was blocked with 5% BLOTTO in TBS for 90 min at RT.The membrane was probed with the primary antibody at a 1:10,000 dilution in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4°C. For detection HRP conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 1 h at 4°C with FemtoMax™ enhanced chemiluminescent reagent.Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemistry of formalin fixed and paraffin embedded using AKT1 (phospho S473) monoclonal antibody, clone 17F6.B11 (Cat # MAB1919) shows detection of Phospho-AKT1 S473 in human prostate tissue.The antibody was used at 20 ug/mL .The staining is much stronger than the weak basal level of phosphorylation in normal prostate tissue.Signal was developed using Dako"s Techmate streptavidin-biotin reagents.Personal communication, Glenna Burmer, Lifespan Biosciences, Seattle, WA.
      • Enzyme-linked Immunoabsorbent Assay
      • Application Image
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • enlarge
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • enlarge
      • Enzyme-linked Immunoabsorbent Assay
      • Gene Information
      • Entrez GeneID:
      • 207
      • Protein Accession#:
      • P31749;AAH00479
      • Gene Name:
      • AKT1
      • Gene Alias:
      • AKT,MGC99656,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA
      • Gene Description:
      • v-akt murine thymoma viral oncogene homolog 1
      • Omim ID:
      • 164730, 181500
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
      • Other Designations:
      • RAC-alpha serine/threonine-protein kinase,murine thymoma viral (v-akt) oncogene homolog-1,protein kinase B,rac protein kinase alpha
      • Interactome
      • Interactome
      • Gene Pathway
      • Acute myeloid leukemia
      • Adipocytokine signaling pathway
      • Apoptosis
      • B cell receptor signaling pathway
      • Chemokine signaling pathway
      • Chronic myeloid leukemia
      • Colorectal cancer
      • Endometrial cancer
      • ErbB signaling pathway
      • Fc epsilon RI signaling pathway
      • Fc gamma R-mediated phagocytosis
      • Focal adhesion
      • Glioma
      • Insulin signaling pathway
      • Jak-STAT signaling pathway
      • MAPK signaling pathway
      • Melanoma
      • mTOR signaling pathway
      • Neurotrophin signaling pathway
      • Non-small cell lung cancer
      • Pancreatic cancer
      • Pathways in cancer
      • Prostate cancer
      • Renal cell carcinoma
      • Small cell lung cancer
      • T cell receptor signaling pathway
      • Tight junction
      • Toll-like receptor signaling pathway
      • VEGF signaling pathway
      • Related Disease
      • Adenocarcinoma
      • Adenocarcinoma, Papillary
      • Alzheimer Disease
      • Alzheimer disease
      • Amphetamine-Related Disorders
      • Atherosclerosis
      • Basal Ganglia Diseases
      • Bipolar Disorder
      • Breast Neoplasms
      • Calcinosis
      • Carcinoma
      • Carcinoma, Non-Small-Cell Lung
      • Carcinoma, Papillary
      • Carcinoma, Papillary, Follicular
      • Cardiovascular Diseases
      • Cognition
      • Colonic Neoplasms
      • Colorectal Neoplasms
      • Coronary Artery Disease
          • ... see more
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴(yán)謹(jǐn)態(tài)度，由螞蟻淘公司專業(yè)人員負(fù)責(zé)品控、采購、物流、銷售、售后，保障正品優(yōu)質(zhì)。以“快速好省，為科研提供好產(chǎn)品、好價(jià)格”為理念，直接鏈接原廠家，從全國各地原制造商嚴(yán)格挑選188款科研精品，剔除品牌溢價(jià)，188生物新電商，把好的產(chǎn)品帶給科研！? 力求給你最優(yōu)質(zhì)的商品。
  • Q：生物188產(chǎn)品正品保障嗎？
    A：生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗(yàn)，在業(yè)界享有良好的口碑；自營商城，直接從廠家采購， 自己的團(tuán)隊(duì)負(fù)責(zé)國際物流和清關(guān)，中間沒有第三方，所有流程嚴(yán)格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進(jìn)行修改；客服電話：4000-520-616

    Q：可以開發(fā)票嗎？
    A：本網(wǎng)站所售商品都是正規(guī)清關(guān)，均開具16%正規(guī)發(fā)票，發(fā)票金額含配送費(fèi)金額，另有說明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內(nèi)送達(dá)！

    Q：如何聯(lián)系商家？
    A：有任何問題夠可以電話咨詢我們，全國24小時(shí)免費(fèi)服務(wù)熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯(cuò)了怎么辦？
    A：同個(gè)訂單購買多個(gè)商品可能會(huì)分為一個(gè)以上包裹發(fā)出，可能不會(huì)同時(shí)送達(dá)，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

    Q：退換貨/維修需要多長時(shí)間？
    A：一般情況下，退貨處理周期為客戶收到產(chǎn)品一個(gè)月內(nèi)（以快遞公司顯示簽收時(shí)間為準(zhǔn)），包裝規(guī)格、 數(shù)量、品種不符，外觀毀損、短缺或缺陷，請?jiān)谑盏截?4小時(shí)內(nèi)申請退換貨；特殊商品以合同條款為準(zhǔn)。

何為188

極簡而嚴(yán)謹(jǐn)，我們僅銷售188款生物醫(yī)學(xué)科研用品，款款都是爆款；因?yàn)樯偎跃劢?，聚焦甄選每一款產(chǎn)品，聚焦服務(wù)每一位客戶!

關(guān)注我們 :

點(diǎn)擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號(hào)

獲取最新優(yōu)惠活動(dòng)通知