ELISA (1:1000)Immunocytochemistry (1:50)Immunofluorescence (1:50)Western Blot (1:1000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).
Storage Instruction:
Store at -20°C.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot
Immunocytochemistry
Immunocytochemical staining of HEK293 with 4-Hydroxynonenal monoclonal antibody, clone 12F7 (HRP) (Cat # MAB17402). (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alex Fluor 633 F-Actin stain. (E, F) 4-Hydroxynonenal Antibody. (G, H) Composite. (A, C, E, G) Untreated and (B, D, F, H) Cells cultured overnight with 50 uM H2O2.