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Millipore/MAB16985 | Anti-MCAM Antibody, clone P1H12/MAB16985/100 µg
  • Millipore/MAB16985 | Anti-MCAM Antibody, clone P1H12/MAB16985/100 µg

Millipore/MAB16985 | Anti-MCAM Antibody, clone P1H12/MAB16985/100 µg

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貨號: MAB16985
品牌: Millipore
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    • Description
      CatalogueNumberMAB16985
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-MCAMAntibody,cloneP1H12
      AlternateNames
      • CD146antigen
      • CellsurfaceglycoproteinP1H12
      • Melanoma-associatedantigenA32
      • Melanoma-associatedantigenMUC18
      • S-endo1endothelial-associatedantigen
      • melanomaadhesionmolecule
      • melanomacelladhesionmolecule
      BackgroundInformationMUC18antigen(CD146),amemberoftheimmuoglobulinsuperfamily,isanintrinsicmembraneglycoproteinof110-120kDafoundonthesurfaceofendothelialcells,bonemarrowfibroblastsandvariousmelanomas.MUC18hasbeenusedasaMarkeroftumorprogressioninhumanmelanomabecauseexpressioninthosetumorscorrelatesstronglywithpoorprognosisandthedevelopmentofmetasticdisease.Inaddition,anumberofhumanT,BandmyeloidleukemiccelllinesseemtoexpressMUC18.TheclosestructuralrelationshipwithN-CAMandrelatedmoleculessuggeststhatMUC18maybealsoadevelopmentallyregulatedcelladhesionmolecule(MelanomaadhesionmoleculeorMCAM).
      ProductInformation
      FormatPurified
      Control
      • HUVECcells.
      PresentationPurifiedmousemonoclonalIgG1inbuffercontaining0.02MPBwith0.25MNaCl,pH=7.6,with0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
      Applications
      ApplicationAnti-MCAMAntibody,cloneP1H12detectslevelofMCAM&hasbeenpublished&validatedforuseinELISA,FC,IC,IH,IP&WB.
      KeyApplications
      • ELISA
      • FlowCytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • WesternBlotting
      ApplicationsNotRecommended
      • Immunohistochemistry(Paraffin)
      ApplicationNotesImmunocytochemistry:
      1-10μg/mLofapreviouslotworkedinimmunocytochemistry.WorksbestonEDTAorTrypsinliftedendothelialcells.

      Immunohistochemistry:
      1-10μg/mL.4%PFAfor30minRTor<2hrs at="" 4°c.="" block="" w/="" 1%bsa/0.2%="" tween20/pbs="" for="" 30min.="" works="" well="" in="" frozen="" tissue;="" fixed="" or="" unfixed.="">

      Immunoprecipitation:
      1-10μg/mLofapreviouslotworkedinimmunoprecipitation.

      ELISA:
      1-10μg/mLofapreviouslotworkedinELISA.

      FACSAnalysis:
      1-10μg/mLofapreviouslotworkedinFACS.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenImmunizationofmicewithHUVEC.
      CloneP1H12
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityP1H12reactsspecificallywithCD146{MCAM,MUC18}.InbloodandmarrowP1H12reactsonlywithendothelialcells,andhasbeenusedtodetectcirculatingendothelialcellsinhuman,dogsandmousesamples.Itpositivelystainsnormal,primaryHUVECandMVECculturesandtheendothelialcellsofallvesselsinnormalfrozensectionsofhumanskin,intestine,ovarytonsil,lymphnode,lung,andkidney.ItdoesnotstaincarcinomacelllinesHT-29andCOLO205,andM21,theTcelllinesJurkatandHuT78,fibroblasts,HL-60orCHOcells,orEBV-transformedBcelllines,althoughitexpectedthatCD146expressionispresentinmanytumorlines.P1H12doesnotstainnormalmonocytes,granulocytes,redcells,platelets,TcellsorBcellsfrommarroworperipheralblood;nordoesitdetectmarrowmegakaryocytesorthemegakaryoblastlineHU3.TheperipheralbloodcellsthatdostainwithP1H12alsoarepositiveforbothvonWillebrandFactor(vWF)andthrombomodulin(thecombinedexpressionofwhichislimitedtoendothelium),andtheystainforfltandflk.Inrecenttesting,melanomaA2058,SKMEL.3andA375.S2arepositive,whilemurinemelanomaM3isnegative.Onesmoothmuscleline(HISM)waspositiveandonenegative(TIGHA-VSMC).Humanbloodlymphoctesarenegativewithorwithoutpriorstimulation.
      IsotypeIgG1
      SpeciesReactivity
      • Canine
      • Human
      • Mouse
      SpeciesReactivityNoteDetectscirculatingendothelialcellsinhuman.Expectedtocross-reactwithdog&mouse.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • MCAM
      • Gicerin
      • MUC18
      • CD146
      Non-ReactiveSpecies
      • Rat
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P43121#Playsaroleincelladhesion,andincohesionoftheendothelialmonolayeratintercellularjunctionsinvasculartissue.Itsexpressionmayallowmelanomacellstointeractwithcellularelementsofthevascularsystem,therebyenhancinghematogeneoustumorspread.Couldbeanadhesionmoleculeactiveinneuralcrestcellsduringembryonicdevelopment.ActsassurfacereceptorthattriggerstyrosinephosphorylationofFYNandPTK2,andatransientincreaseintheintracellularcalciumconcentration.
      SIZE:646aminoacids;71607Da
      SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
      TISSUESPECIFICITY:Detectedinendothelialcellsinvasculartissuethroughoutthebody.Mayappearatthesurfaceofneuralcrestcellsduringtheirembryonicmigration.Appearstobelimitedtovascularsmoothmuscleinnormaladulttissues.Associatedwithtumorprogressionandthedevelopmentofmetastasisinhumanmalignantmelanoma.ExpressedmoststronglyonmetastaticlesionsandadvancedprimarytumorsandisonlyrarelydetectedinbenignmelanocyticneviandthinprimarymelanomaswithalowprobABIlityofmetastasis.
      SIMILARITY:SwissProt:P43121##Contains3Ig-likeC2-type(immunoglobulin-like)domains.&Contains2Ig-likeV-type(immunoglobulin-like)domains.
      MolecularWeight110kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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