7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit is a fluorescence-labeled method for determining the cytotoxic effect of immune effector cells at a single cell level.
Storage Instruction:
Store the kit at -20°C temporarily. For best results, store the components as described in the protocol.
Protocol:
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MSDS:
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Regulation Status:
For research use only (RUO)
Datasheet:
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Applications
Flow Cytometry
The degree of cytotoxicity of natural killer cells (effector cells; NK cells) on K-562 cells (target cells) depends on the ratio of NK cells to K-562 cells. K-562 cells were labeled with CFSE and co-cultured with NK cells at the ratios indicated above. Cells were co-cultured for four hours at 37°C. At the end of the experiment, dead cells were labeled with 7-AAD. The percentage of K-562 cell death was analyzed by a flow cytometer. When K-562 cells were cultured alone, there is little cell death (2%). Addition of NK cells at a ratio as low as 6.25 : 1 caused a significant increase in K-562 cell death (8.2%). At a ratio of NK cells to target cells of 25 : 1, there is 19.7% death of K-562 cells.
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