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Abnova/ACTB monoclonal antibody (M01), clone 3G4-F9/1kit/H00000060-M01
  • Abnova/ACTB monoclonal antibody (M01), clone 3G4-F9/1kit/H00000060-M01

Abnova/ACTB monoclonal antibody (M01), clone 3G4-F9/1kit/H00000060-M01

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貨號: H00000060-M01
品牌: Abnova
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  • 詳情
  • 使用說明
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      • Specification
      • Product Description:
      • Mouse monoclonal antibody raised against a full length recombinant ACTB.
      • Immunogen:
      • ACTB (AAH01301, 1 a.a. ~ 375 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
      • Sequence:
      • MDDDIAALVVDNGSGMCKAGFAGDDAPRAVSPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERRYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
      • Host:
      • Mouse
      • Reactivity:
      • Human, Mouse
      • Isotype:
      • IgG1 Kappa
      • Quality Control Testing:
      • Antibody Reactive Against Recombinant Protein.QC Testing of H00000060-M01Western Blot detection against Immunogen (66.99 KDa) .
      • Storage Buffer:
      • In 1x PBS, pH 7.4
      • Storage Instruction:
      • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
      • MSDS:
      • PDF DownloadDownload
      • Datasheet:
      • PDF DownloadDownload
      • Publication Reference
      • 1.
      • Propargylglycine decreases neuro-immune interaction inducing pain response in temporomandibular joint inflammation model.Garattini EG, Santos BM, Ferrari D, Porto C, Francescato HDC, Coimbra TM, Leite-Panissi CRA, Branco LGS, Nascimento GC.Nitric Oxide. 2019 Dec 1;93:90-101. doi: 10.1016/j.niox.2019.10.001. Epub 2019 Oct 8.
      • 2.
      • Monospecific antibody targeting of CDH11 inhibits epithelial-to-mesenchymal transition and represses cancer stem cell-like phenotype by up-regulating miR-335 in metastatic breast cancer, in vitro and in vivo.Chen JH, Huang WC, Bamodu OA, Chang PM, Chao TY, Huang TH.BMC Cancer. 2019 Jun 27;19(1):634. doi: 10.1186/s12885-019-5811-1.
      • 3.
      • MiR-9 functions as a tumor suppressor in acute myeloid leukemia by targeting CX chemokine receptor 4.Zhu B, Xi X, Liu Q, Cheng Y, Yang H.Am J Transl Res. 2019 Jun 15;11(6):3384-3397. eCollection 2019.
      • 4.
      • Quinacrine pretreatment reduces microwave-induced neuronal damage by stabilizing the cell membrane.Ding XF, Wu Y, Qu WR, Fan M, Zhao YQ.Neural Regen Res. 2018 Mar;13(3):449-455
      • 5.
      • Mammalian Oocytes Locally Remodel Follicular Architecture to Provide the Foundation for Germline-Soma Communication.El-Hayek S, Yang Q, Abbassi L, FitzHarris G, Clarke HJ.Curr Biol. 2018 Apr 2;28(7):1124-1131.e3. doi: 10.1016/j.cub.2018.02.039. Epub 2018 Mar 22.
      • 6.
      • Multiple chimeric antigen receptors successfully target chondroitin sulfate proteoglycan 4 in several different cancer histologies and cancer stem cells.Beard RE, Zheng Z, Lagisetty KH, Burns WR, Tran E, Hewitt SM, Abate-Daga D, Rosati SF, Fine HA, Ferrone S, Rosenberg SA, Morgan RAJ Immunother Cancer. 2014 Aug 19;2:25. doi: 10.1186/2051-1426-2-25. eCollection 2014.
      • 7.
      • A microRNA-135a/b binding polymorphism in CD133 confers decreased risk and favorable prognosis of lung cancer in Chinese by reducing CD133 expression.Cheng M, Yang L, Yang R, Yang X, Deng J, Yu B, Huang D, Zhang S, Wang H, Qiu F, Zhou Y, Lu JCarcinogenesis. 2013 May 28.
      • 8.
      • Systematic proteomic analysis of human hepotacellular carcinoma cells reveals molecular pathways and networks involved in metastasis.Yu Y, Shen H, Yu H, Zhong F, Zhang Y, Zhang C, Zhao J, Li H, Chen J, Liu Y, Yang P.Mol Biosyst. 2011 Jun;7(6):1908-16. Epub 2011 Apr 6.
      • 9.
      • Identification of early transcripts related to male development in chicken embryos.Lin YP, Chen LR, Chen CF, Liou JF, Chen YL, Yang JR, Shiue YL.Theriogenology. 2010 Aug 20. [Epub ahead of print]
      • 10.
      • Anti-microtubule "plinabulin" chemical probe KPU-244-B3 labeled both alpha- and beta-tubulin.Yamazaki Y, Sumikura M, Hidaka K, Yasui H, Kiso Y, Yakushiji F, Hayashi Y.Bioorg Med Chem. 2010 Mar 23. [Epub ahead of print]
      • 11.
      • 7beta-Hydroxy-epiandrosterone-mediated regulation of the prostaglandin synthesis pathway in human peripheral blood monocytes.Mee SL, Hennebert O, Ferrec C, Wulfert E, Morfin R.Steroids. 2008 Oct;73(11):1148-59. Epub 2008 May 14.
      • Applications
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • ACTB monoclonal antibody (M01), clone 3G4-F9 Western Blot analysis of ACTB expression in HeLa ( Cat # L013V1 ).
      • PDF DownloadProtocol Download
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • ACTB monoclonal antibody (M01), clone 3G4-F9. Western Blot analysis of ACTB expression in Raw 264.7 ( Cat # L024V1 ).
      • PDF DownloadProtocol Download
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • ACTB monoclonal antibody (M01), clone 3G4-F9. Western Blot analysis of ACTB expression in NIH/3T3 ( Cat # L018V1 ).
      • PDF DownloadProtocol Download
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • ACTB monoclonal antibody (M01), clone S1. Western Blot analysis of ACTB expression in K-562.
      • PDF DownloadProtocol Download
      • Western Blot (Transfected lysate)
      • Western Blot (Transfected lysate)
      • Western Blot analysis of ACTB expression in transfected 293T cell line by ACTB monoclonal antibody (M01), clone 3G4-F9.Lane 1: ACTB transfected lysate(42 KDa).Lane 2: Non-transfected lysate.
      • PDF DownloadProtocol Download
      • Western Blot (Recombinant protein)
      • PDF DownloadProtocol Download
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)enlargeenlarge this image
      • Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml]
      • PDF DownloadProtocol Download
      • Sandwich ELISA (Recombinant protein)
      • Sandwich ELISA (Recombinant protein)
      • Detection limit for recombinant GST tagged ACTB is approximately 1ng/ml as a capture antibody.
      • PDF DownloadProtocol Download
      • ELISA
      • Application Image
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • enlarge
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • enlarge
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • enlarge
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • enlarge
      • Western Blot (Transfected lysate)
      • Western Blot (Transfected lysate)
      • enlarge
      • Western Blot (Recombinant protein)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • enlarge
      • Sandwich ELISA (Recombinant protein)
      • Sandwich ELISA (Recombinant protein)
      • enlarge
      • ELISA
      • Gene Information
      • Entrez GeneID:
      • 60
      • GeneBank Accession#:
      • BC001301
      • Protein Accession#:
      • AAH01301
      • Gene Name:
      • ACTB
      • Gene Alias:
      • PS1TP5BP1
      • Gene Description:
      • actin, beta
      • Omim ID:
      • 102630
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
      • Other Designations:
      • PS1TP5-binding protein 1,actin, cytoplasmic 1,beta actin,beta cytoskeletal actin
      • Gene Pathway
      • Adherens junction
      • Arrhythmogenic right ventricular cardiomyopathy (ARVC)
      • Focal adhesion
      • Hypertrophic cardiomyopathy (HCM)
      • Leukocyte transendothelial migration
      • Pathogenic Escherichia coli infection - EHEC
      • Regulation of actin cytoskeleton
      • Tight junction
      • Vibrio cholerae infection
      • Related Disease
      • Breast cancer
      • Breast Neoplasms
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