mutaFISH™ BRAF V600E V600wt RNA Probes is designed to detect human BRAF V600E gene mutation on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
Comparison:
FISH Probes vs mutaFISH™ Probes
Fluorophore:
Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)Texas Red X (Excitation Peak (nm): 595; Emission Peak613)
Probe Position:
Regulatory Status:
For research use only (RUO)
Video:
Datasheet:
Download
Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human Sk-HEP1 cells. BRAF V600E point mutation was detected via orange signal (Cy3), BRAF V600wt was detected via red signal (Cy5).
mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
mutaFISH™ staining was performed in situ in mouse FFPE BRAF 293T tissue. BRAF V600wt was detected via red signal (Texas Red X).
Application Image
mutation specific, Fluorescence In Situ Hybridization (Cells)
enlarge
mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
enlarge
Gene Information
Entrez GeneID:
673
Gene Name:
BRAF
Gene Alias:
B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1
Gene Description:
v-raf murine sarcoma viral oncogene homolog B1
Omim ID:
115150, 164757, 211980
Gene Ontology:
Hyperlink
Gene Summary:
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
購物車為空，快去購物吧！
4000-520-616
咨詢顧問
特別 提示
Protocol Download
