BackgroundInformation | Bromodeoxyuridine(BrdU)isathymidineanalogandisspecificallyincor-poratedintoDNAduringDNAsynthesis.Anti-bromodeoxyuridinemonoclonalantibodyisusedtoidentifycellsthathaveincorporatedBrdU.ThisimmunologicaldetectionschemehasseveraladvantagesovertheuseofrADIoactivethymidineincorporationforidentifyingcellsunder-goingreplication.Labelinganddetectioncanbeperformedthesamedayinsteadofwaitingseveraldays,asrequiredforautoradiographyoftritium-labeledcells,andthenecessityofusingmultiplespecimensforobtainingtheoptimalexposuretimeiseliminated.Inaddition,anti-bromodeoxyuridinestainingwithflowcytometricanalysisallowsmultipleparameterstobeevaluatedsimultaneously.ThisantibodydoesnotrequirepretreatmentofcellswithstrongDNAdenaturantsinorderfortheantibodytorecognizeincorporatedBrdU.Theantibody"sspecialABIlityisthoughttobeduetothepresenceofDNAseintheantibodysolutionarisingfromthemycoplasmainfectingtheoriginalhybridomaline{Gonchoroff,1985}. |