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Millipore/FCCS025105 | FlowCellect? PI3K Activation Dual Detection Kit/FCCS025105/25 Tests / Kit
  • Millipore/FCCS025105 | FlowCellect? PI3K Activation Dual Detection Kit/FCCS025105/25 Tests / Kit

Millipore/FCCS025105 | FlowCellect? PI3K Activation Dual Detection Kit/FCCS025105/25 Tests / Kit

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貨號: FCCS025105
品牌: Millipore
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    • Description
      CatalogueNumberFCCS025105
      TradeName
      • FlowCellect
      DescriptionFlowCellect?PI3KActivationDualDetectionKit
      OverviewMillipore’sFlowCellect?PI3KActivationDualDetectionkitincludestwodirectlyconjugatedantibodies,aphospho-specificAnti-phospho-Akt(Ser473)-AlexaFluor?488andanAnti-Akt-AlexaFluor?647conjugatedantibodytomeasuretotallevelsofAkt.ThistwocolorflowcytometrykitisdesignedtomeasuretheextentofAktphosphorylationrelativetothetotalAktexpressioninanygivencellpopulation.Bydoingsuch,thelevelsofboththetotalandphosphorylatedproteincanbemeasuredsimultaneouslyinthesamecell,resultinginanormalizedandaccuratemeasurementofAktactivationafterstimulation.Moreover,simultaneousmeasurementofbothtotalandphospho-Aktconfirmstargetspecificityofthephosphorylationevent.Together,atotalandphosphoantibodyduoperformedinmultiplexprovidesanenhancedandmorereliabledetectionofthephospho:totalratiowithinamixedpopulation.

      AllFlowCellectkitsaresuppliedwithoptimizedfixation,permeABIlization,wash,andassaybufferstoprovideresearcherstheabilitytodetecttheactivationofAktinflowapplications.Sufficientreagentsareprovidedfor25two-colorsamples.Thekitincludesalloptimizedfluorescentlylabeledantibodiesandbuffersnecessaryforcellpreparationandanalysis.
      AlternateNames
      • FlowCellect
      BackgroundInformationAkt/PKBisaSer/ThrkinaseandamajorknowneffecterofthePI3Kinasepathway.ItisinvolvedinmultiplesignalingpathwaysthatrelatetomanyBIOLOGicalprocessesincludingmetabolism,apoptosis,cellcyclecontrol,angiogenesis,differentiation,andcellgrowthandproliferation.Inmammals,threeisoformsofAkt(Akt1/PKCα,Akt2/PKBβ,andAkt3/PKBγ)exists.Theyexhibitahighdegreeofhomology,butdifferslightlyinthelocalizationoftheirregulatoryphosphorylationsites.Akt1isthepredominantisoformthatisinmosttissuesandisthoughttohaveadominantroleingrowth,survival,embryonicdevelopment,andADIpocytedifferentiation.Akt2iscorrelatedwiththeregulationofglucosehomeostasisandisthepredominantisoformexpressedininsulin-responsivetissues.Akt3isabundantinbraintissue.EachAktisoformiscomposedofthreefunctionallydistinctregions:anN-terminalPleckstrinHomology(PH)domainthatprovidesalipid-bindingmodule,acentralcatalyticdomaincontainingThr308,andaC-terminalhydrophobicmotifcontainingSer473.TheactivationofAKTisdependentonadualregulatorymechanismthatrequiresbothitstranslocationtotheplasmamembraneanddualphosphorylationonThr308andSer473byPDK1andtheTORC2complex,respectively.

      PhosphorylationofAktatSer473servesasamajorsignalingnodetoactivateitsmanydownstreameffectorssuchasmTOR,Bad,GSK3,Forkhead,andCaspases,leadingtomultiplebiologicalprocessessuchascellsurvival,cellgrowth,andcellmetabolism.
      ProductInformation
      Components
      • 1.20XAnti-phospho-Akt(Ser473)AlexaFluor?488ConjugatedAntibody:(PartNo.CS203310)Onevialcontaining150μL.
      • 2.20XAnti-Akt/PKB-AlexaFluor?647ConjugatedAntibody:(PartNo.CS205073)Onevialcontaining150μL.
      • 3.FixationBuffer:(PartNo.CS202122)Onebottlecontaining13mL.
      • 4.10XWashBuffer:(PartNo.CS202123)Onebottlecontaining13mL.
      • 5.5XAssayBuffer:(PartNo.CS202124)One55mLbottle
      • 6.1XPermeabilizationBuffer:(PartNo.CS203284)Onebottlecontaining13mL.
      DetectionmethodFluorescent
      StorageandShippingInformation
      StorageConditions4-8°Cforantibodiesandbuffers
      Applications
      ApplicationThisFlowCellectPI3KActivationDualDetectionKitforFlowCytometryincludes2conjugatedantibodies,aphospho-specificAnti-phospho-Akt(Ser473)-AlexaFluor488&anAnti-Akt-AlexaFluor647conjugatedantibodytomeasuretotallevelsofAkt.
      KeyApplications
      • FlowCytometry
      BiologicalInformation
      HostMouse
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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