Overview | Celladhesionplaysamajorroleincellularcommunicationandregulation,andisoffundamentalimportanceinthedevelopmentandmaintenanceoftissues.Scientistsarecontinuallyexaminingtheadhesionandmigrationofmanydiversecelltypesonvariousextracellularmatrix(ECM)componentproteins.Millicoat?CellAdhesionStripsareprovidedas128-wellremovablestripsinaplateframeforconvenienceandflexibilityindesigningassays.ThewellsinrowsA-GhavebeencoatedwithahumanECMprotein.RowHofeachstripiscoatedwithBSAwhichservesasanegativeassaycontrol.TheMillicoatTMscreeningkitcontainsindividual96-wellplatesforfibronectin,vitronectin,laminin,collagenIandcollagenIV.Cellsareseededontothecoatedsubstrate.Subsequently,adherentcellsarefixedandstained.RelativeattachmentisdeterminedusingabsorbancereADIngs. |