Description:
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TheNEBNextUltraIIEndRepair/dA-TailingModulehasbeenoptimizedtoconvert500pg-1μgoffragmentedDNAtorepairedDNAhaving5′phosphorylated,3′dA-tailedends.ThemoduleisoptimizedforusewiththeNEBNextUltraIILigationModule(NEB#E7595),andispartoftheUltraIIworkflowwhichenableshighyieldpreparationofhighqualitylibrariesfrom500picogramsto1microgramofinputDNA.
ThismoduleiscompatIBLewithIlluminaworkflowsandwithsomeOxfordNanoporeMinION?workflows.FunctionalValidation
EachsetofreagentsisfunctionallyvalidatedtogetherthroughconstructionandsequencingofatranscriptomelibraryandsequencedonaDNAlibraryontheIlluminasequencingplatform.
LotControl
Thelotsprovidedaremanagedseparatelyandqualifiedbyadditionalfunctionalvalidation.Individualreagentsundergostandardenzymeactivityandqualitycontrolassays,andalsomeetstringentcriteriaintheadditionalqualitycontrolslistedoneachindividualcomponentpage
ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
NEBNextUltraIIEndPrepEnzymeMix | -20 | |
NEBNextUltraIIEndPrepReactionBuffer | -20 | |