當(dāng)前位置 : NEB >>> NEB/NEBNext? Ultra? II DNA PCR-free Library Prep with Sample Purification Beads/24 reactions/E7415S
NEB/NEBNext? Ultra? II DNA PCR-free Library Prep with Sample Purification Beads/24 reactions/E7415S
  • NEB/NEBNext? Ultra? II DNA PCR-free Library Prep with Sample Purification Beads/24 reactions/E7415S

NEB/NEBNext? Ultra? II DNA PCR-free Library Prep with Sample Purification Beads/24 reactions/E7415S

價(jià)格: 試用 市場價(jià): 0.00

貨號(hào): E7415S
品牌: NEB
規(guī)格
數(shù)量
庫存(0)
特別 提示
代購產(chǎn)品:無質(zhì)量問題不接受退換貨,下單前請(qǐng)仔細(xì)核對(duì)信息。
下單后請(qǐng)及時(shí)聯(lián)系客服核對(duì)商品價(jià)格,訂單生效后再付款。
資深產(chǎn)品顧問
咨詢顧問

全國免費(fèi)服務(wù)熱線

4000-520-616


  • 自營商城 一站式服務(wù)
  • 廠家直采 剔除溢價(jià)
  • 品質(zhì)甄選 正品保證
  • 嚴(yán)控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說明
  • 常見問題
    • The NEBNext Ultra II DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of sheared DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step. The fast, user-friendly workflow requires minimal hands-on time, resulting in excellent library yields and GC coverage without requiring PCR.

      For added convenience, this kit contains NEBNext Sample Purification Beads (SPRIselect beads from Beckman Coulter) for size selection and enzyme reaction clean-up steps.Advantages

      • Enjoy flexible input amounts from 250 ng to 1,000 ng of sheared DNA
      • Get more of what you need with reliably high library yields
      • Prepare PCR-free libraries with a broad range of sample types
      • Save time with streamlined workflows, reduced hands-on time, and automation compatibility
      • Use with the NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1)or other Illumina-compatible index adaptors with a 3’ single T overhang

      A separate version of the NEBNext Ultra II DNA PCR-free Library Prep Kit is available without sample purification beads.

      Figure 1: NEBNext Ultra II DNA PCR-free Library Prep Kit generates libraries with higher yields
      A. PCR-free libraries were prepared with NA19240 genomic DNA (Coriell Institute) using NEBNext Ultra II DNA PCR-free and Illumina TruSeq® PCR-free library prep kits and size selected for 350 bp inserts. DNA inputs were 1 µg.B. Libraries of 150-200 bp inserts were prepared using NEBNext Ultra II DNA PCR-free and Roche Sequencing Kapa HyperPrep library prep kits coupled with Covaris® shearing without size selection. NEBNext Unique Dual Index UMI Adaptors DNA Set 1, IDT for Illumina (TruSeq DNA UD Indexes) and Kapa Dual-Indexed Adaptors were used for the NEBNext, Illumina, and Kapa kits, respectively, following manufacturers" recommendations.
      Figure 2: NEBNext Ultra II DNA PCR-free Library Prep provides uniform genome coverage with human DNA
      NEBNext Ultra II DNA PCR-free libraries have even more genome coverage than Illumina DNA PCR-free libraries. Sequencing libraries were prepared according to the manufacturer’s recommendations from 250 ng of NA19240 cell line DNA and sequenced on a single NovaSeq 6000 S4 v1.5 flow cell (PE 2x150 bp). Reads were adaptor trimmed (fastp 0.20.0), aligned to the Telomere-to-Telomere chm13 reference (draft 1, bwa-mem 0.7.17), and duplicate marked (samblaster 0.1.24). 2.8B reads were randomly sampled from each bam (sambamba view -s 0.6.8) Coverage of primary, pass-filter assignments was assessed using mosdepth (v0.3.1, -F 772) and plotted for autosomes or the mitochondrial genome (inset). Expected coverage (num_reads*read length/genome size) is indicated).
      This product is related to the following categories:
      DNA Library Prep for Illumina,
      Automation for NEBNext? NGS Library Prep,
      Next Generation Sequencing Library Preparation
    售后保障
    螞蟻淘生物188,秉承螞蟻淘一貫的嚴(yán)謹(jǐn)態(tài)度,由螞蟻淘公司專業(yè)人員負(fù)責(zé)品控、采購、物流、銷售、售后,保障正品優(yōu)質(zhì)。以“快速好省,為科研提供好產(chǎn)品、好價(jià)格”為理念,直接鏈接原廠家,從全國各地原制造商嚴(yán)格挑選188款科研精品,剔除品牌溢價(jià),188生物新電商,把好的產(chǎn)品帶給科研!? 力求給你最優(yōu)質(zhì)的商品。
  • Q:生物188產(chǎn)品正品保障嗎?
    A:生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗(yàn),在業(yè)界享有良好的口碑;自營商城,直接從廠家采購, 自己的團(tuán)隊(duì)負(fù)責(zé)國際物流和清關(guān),中間沒有第三方,所有流程嚴(yán)格把控,100%保證正品,假一罰十。

    Q:下單后可以修改訂單嗎?
    A:下單后的商品付款之前可以修改;訂單付款成功,需要聯(lián)系我們客服進(jìn)行修改;客服電話:4000-520-616

    Q:可以開發(fā)票嗎?
    A:本網(wǎng)站所售商品都是正規(guī)清關(guān),均開具16%正規(guī)發(fā)票,發(fā)票金額含配送費(fèi)金額,另有說明的除外。

    Q:商品幾天可以發(fā)貨?
    A:生物188商品,全部現(xiàn)貨銷售,付款后即可發(fā)貨,一般一周內(nèi)送達(dá)!

    Q:如何聯(lián)系商家?
    A:有任何問題夠可以電話咨詢我們,全國24小時(shí)免費(fèi)服務(wù)熱線:4000-520-616 或聯(lián)系我們的在線客服QQ:1570468124

    Q:收到的商品少了/發(fā)錯(cuò)了怎么辦?
    A:同個(gè)訂單購買多個(gè)商品可能會(huì)分為一個(gè)以上包裹發(fā)出,可能不會(huì)同時(shí)送達(dá),建議查看訂單詳情是否是 部分發(fā)貨狀態(tài);如未收到,可聯(lián)系在線客服或者致電4000-520-616。

    Q:退換貨/維修需要多長時(shí)間?
    A:一般情況下,退貨處理周期為客戶收到產(chǎn)品一個(gè)月內(nèi)(以快遞公司顯示簽收時(shí)間為準(zhǔn)),包裝規(guī)格、 數(shù)量、品種不符,外觀毀損、短缺或缺陷,請(qǐng)?jiān)谑盏截?4小時(shí)內(nèi)申請(qǐng)退換貨;特殊商品以合同條款為準(zhǔn)。

何為188

極簡而嚴(yán)謹(jǐn),我們僅銷售188款生物醫(yī)學(xué)科研用品,款款都是爆款;因?yàn)樯偎跃劢?,聚焦甄選每一款產(chǎn)品,聚焦服務(wù)每一位客戶!

關(guān)注我們 :

點(diǎn)擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號(hào)

獲取最新優(yōu)惠活動(dòng)通知