DENGUE VIRUS SEROTYPE 3 VLP

Dengue Virus Serotype 3 VLP (Virus-Like Particle) is a new, unique product that has been developed in response to the need for highly immunogenic, concentrated Dengue antigens for use in Dengue focussed disease research and vaccine development (including use as an immunogen).

PRODUCT DETAILS – DENGUE VIRUS SEROTYPE 3 VLP

• VLPs are constructed from E, M and preM proteins (NCBI Accession Number: AXX75610.1, strain: Sri Lanka D3/H/IMTSSA-SRI/2000/1266), with secretion from the cell aided by substituting the C terminal 20% with the corresponding Japanese Encephalitis Virus sequence (all internal/transmembrane).
• Produced in HEK293 expression system and tested for stability.
• Buffered in 10mM sodium phosphate, 20mM sodium citrate, 154mM sodium chloride, pH7.4.
• Concentration and purification by a series of ultracentrifugation and chromatographical methods which result in VLPs of exceptional quality and purity.
• Cleavage of preM protein occurs naturally by furin in the cell and results in a dual population of preM and M-containing VLPs.

BACKGROUND

Virus-Like Particles (VLPs) are an emerging vaccine technology. VLPs consist of protein shells comprising outer proteins specific to the virus in question. VLPs are more representative of how viral antigens are presented in vivo and while they are highly immunogenic they are non-infectious as they lack the core genetic material of the virus. Another important advantage of VLPs is that they more effectively activate key aspects of the immune response to achieve potent immune stimulation and to provide immunological memory. VLP-based vaccines have also shown to provide effective protection and are in current use for several diseases and in development for many others.

With more than one-third of the world’s population living in areas at risk of transmission, dengue infection is a leading cause of illness and death in the tropics and subtropics (ref WHO for Guidelines for Diagnosis, Treatment, Prevention & Control, 2009) As many as 100 million people are infected yearly. Dengue is caused by any one of four related viruses transmitted by mosquitoes. There are no vaccines available to prevent infection with dengue virus. Dengue Virus Serotype 1, Serotype 2 and Serotype 4 VLPs are now also available.