當前位置 ： Millipore >>> Millipore/DAB500 | IHC Select? HRP/DAB, 500 Tests/DAB500/1 kit500 tests
Millipore/DAB500 | IHC Select? HRP/DAB, 500 Tests/DAB500/1 kit500 tests
  • Millipore/DAB500 | IHC Select? HRP/DAB, 500 Tests/DAB500/1 kit500 tests

Millipore/DAB500 | IHC Select? HRP/DAB, 500 Tests/DAB500/1 kit500 tests

價格: 試用 市場價: 0.00
貨號: DAB500
品牌: Millipore
規(guī)格
數量
庫存(0)
特別 提示
代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯(lián)系客服核對商品價格，訂單生效后再付款。
資深產品顧問
咨詢顧問

全國免費服務熱線

4000-520-616

  • 自營商城 一站式服務
  • 廠家直采 剔除溢價
  • 品質甄選 正品保證
  • 嚴控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說明
  • 常見問題
    • Description
      CatalogueNumberDAB500
      ReplacesDAB250
      BrandFamilyChemicon®
      TradeName
      • IHCSelect
      • Chemicon
      DescriptionIHCSelect?HRP/DAB,500Tests
      OverviewSummaryandExplanation:

      Immunohistochemicaltechniqueshavebeeninuseasadjunctivemethodsfortherecognitionofcellsintissuesectionsforatleast50years.Thefirstmethodreporteduseoffluorescentlabelsin19421.In1974,reportsofusingenzymaticlabels,suchashorserADIshperoxidase(HRP)inroutineH&Eparaffinsectionsappeared2,3.Themethodshavesincebecomethe"standardofcare"insurgicalpathologywhenclassicmethodsalonefailtoyieldadefinitivediagnosis4-9.

      Earlytechniqueswerebasedontheperoxidase-anti-peroxidase(PAP)reaction28,whileimprovementsexploitedthestrongaffinityofavidinforbiotin10.ThistechniqueusesHRP-labeledstreptavidin.Thesecondaryantibodyisconjugatedwithbiotinandthestreptavidin-HRPcomplexreactswiththebiotinonthesecondaryantibodies.Theresultingbiotin-avidin-HRPcomplexcanreactwiththeprimaryantibodyboundtothespecificepitopeofthetargetantigen.TheHRPenzymesofthestreptavidincomplexthencatalyzethesubstrate/chromogenreactiontoformacoloredreactionproduct(browntoblackwhenusingDABasthechromogen)attheantigensite.ABIOLOGicalstainisthenusedtovisualizethewholetissuesection.
      ProductInformation
      Components
      • *20773/50mL-BlockingReagent:normalgoatseruminphosphatebufferedsaline(PBS)containingcarrierprotein
      • *20775/50mL-SecondaryAntibodies:biotinylatedgoatanti-mouseIgGandgoatanti-rabbitIgGinPBS,containingcarrierprotein
      • *20774/50mL-StreptavidinHRP:DilutedinTrisBufferedSaline
      • *71897/4mL-DABChromogenA:3,3"DiaminobenzidinedilutedinTBS
      • *71898/2x50mL-DABChromogenB:hydrogenperoxidedilutedinTBS,containingTween?20
      • *20785/50mL-Hematoxylin:Mayer"shematoxylincounterstain
      • *20784/2x500mL-20XRinseBuffer:TBS
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditionsStABIlityandStorage:

      Whenstoredat2-8°C,theIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemisstableuptotheexpirationdateprintedonthekitlabel.Donotfreezeorexposetoelevatedtemperatures.Discardanyremainingreagentsaftertheexpirationdate.Donotstorekitcomponentsorperformstaininginstronglight,suchasdirectsunlight.

      Precautions:

      ·Pooling,substitutionoralterationofanyreagentmaycauseerroneousresults.

      *Donotmixreagentsfromdifferentlots.

      *Donotallowtheslidestodryatanytimeduringthestainingprocedure.

      *Handleallspecimensandmaterialscomingincontactwiththemaspotentiallyinfectiousmaterials.Disinfectwith0.05%sodiumhypochlorite.

      *DonotmouthPipettereagents.Ifingested,seekprofessionalassistanceorcontactaPoisonControlCenterimmediately.

      ·Diaminobenzidine(DAB)maybecarcinogenic.Thissolutionmaycauseirritationuponskincontact.Avoidinhalation,ingestion,orskincontactandweargloveswhenhandlingDAB.Ifskincontactoccurs,flushaffectedareawithwater.Spillsmaybe"neutralized"withbleach25,26.Disposeofaccordingtolocalregulations.

      ·Inhalationoringestionofxyleneorthehighlyallergenicfixativeformaldehydeisharmful.Thesematerialsshouldbehandledwithgloves.Prepareinafumehood.Ifskinoreyecontactoccurs,washthoroughlywithcopiousamountsofwater.

      ·Weardisposablegloveswhenhandlinganyhumanmaterialandwhileperformingthestainingprocedure.MaterialsRequired:
      ·PrimaryrabbitormouseIgGantibodies(suchasCHEMICONIHCSelect?PredilutedPrimaryAntibodies)
      ·Positiveandnegativetissuecontrolslides
      ·Positivelychargedmicroscopeslides
      ·Glasscoverslips(24x40mm,No.1)
      ·Distilledordeionizedwater
      ·Tween?20
      ·Permanentmountingmedium,Eukitt?AmericanHistologyorequivalent
      ·3%laboratorygradehydrogenperoxide
      ·10.0%neutralbufferedformalin15
      ·Xylene,ACSgrade
      ·Ethanol,absolute(100%)
      ·Latex,rubberor100%nitrilegloves(xyleneresistant)
      ·Timer
      ·Fumehood
      ·LightmicroscopeequippedwithPlanAchromatorequivalentlenses
      ·60°Cdryingoven
      Applications
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      ApplicationNotesTheIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemisintendedforusewiththeCHEMICONIHCSelect?PredilutedPrimaryAntibodyreagentswhichcontainrabbitandmouseIgGforthequalitativeidentificationofantigensbylightmicroscopyinparaffin-embeddedtissues.TheIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemmayalsobeusedwithotherrabbitormouseIgGprimaryantibodies.

      GeneralPurposeReagents
      BiologicalInformation
      SpeciesReactivity
      • Mouse
      • Rabbit
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態(tài)度，由螞蟻淘公司專業(yè)人員負責品控、采購、物流、銷售、售后，保障正品優(yōu)質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優(yōu)質的商品。
  • Q：生物188產品正品保障嗎？
    A：生物188質量把控人員具有十年的從業(yè)經驗，在業(yè)界享有良好的口碑；自營商城，直接從廠家采購， 自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進行修改；客服電話：4000-520-616

    Q：可以開發(fā)票嗎？
    A：本網站所售商品都是正規(guī)清關，均開具16%正規(guī)發(fā)票，發(fā)票金額含配送費金額，另有說明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內送達！

    Q：如何聯(lián)系商家？
    A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯了怎么辦？
    A：同個訂單購買多個商品可能會分為一個以上包裹發(fā)出，可能不會同時送達，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

    Q：退換貨/維修需要多長時間？
    A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規(guī)格、 數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

何為188

極簡而嚴謹，我們僅銷售188款生物醫(yī)學科研用品，款款都是爆款；因為少所以聚焦，聚焦甄選每一款產品，聚焦服務每一位客戶!

關注我們 :

點擊QQ聯(lián)系我們
生物188微信

關注188微信公眾號

獲取最新優(yōu)惠活動通知

  • 品質甄選，正品保證

  • 自營電商，廠家直采

  • 極簡主義，188精品