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Millipore/CBL404 | Anti-p53 Antibody, aa 211-220, clone240/CBL404/100 µg
  • Millipore/CBL404 | Anti-p53 Antibody, aa 211-220, clone240/CBL404/100 µg

Millipore/CBL404 | Anti-p53 Antibody, aa 211-220, clone240/CBL404/100 µg

價格: ￥3204.00 市場價: 5340.00
貨號: CBL404
品牌: Millipore
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    • Description
      CatalogueNumberCBL404
      Replaces04-1083
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-p53Antibody,aa211-220,clone240
      BackgroundInformationp53wasdiscoveredin1979asacellularproteinassociatingwiththetransformingproteinofSV40tumorvirus.Sincethen,manydifferentbiochemicalfunctionshavebeenattributedtothe53kDphosphoprotein.Experimentalevidencehassuggestedthatp53actsasanegativeregulatorofcellgrowthinnormalcells(Finlay,1989).Thus,theinactivationormutationofp53maybeanessentialstepinthedevelopmentofmalignancy(LaneandBenchmol,1990).Wild-typep53levelsinnormalcellsandtissueswerefoundtobeverylow.Mutantp53polypeptide,however,isoftenfoundtobepresentathighconcentrationsinmammaliantumorsandtumorcelllines.Forexample,inanimmuno-histochemistrystudy40%ofhumanbreastcancershowedelevatedlevelsofmutantp53inthecellnucleus.Mutationsofthep53proteinhavesomecharacteristicfeatures:

      a)Mostofthemaremissensepointmutationsgivingrisetoanalteredproteinfunction.

      b)Many-butnotall-mutantp53proteinsexhibitacommonmutantstructure,whichcanberecognizedbymonoclonalantibodiesspecificforp53inthemutantconformation.
      ProductInformation
      FormatPurified
      PresentationPurifiedmousemonoclonalinbuffercontaining0.1MTris-glycine(pH7.4),0.15MNaCl,with0.05%sodiumazide.Werecommendthateachlaboratorydetermineanoptimumworkingtitreforuseinitsparticularapplication.
      StorageandShippingInformation
      StorageConditionsForusewithin1monthofpurchasestoreat+4°C,forlongtermstoragealiquotantibodyintosmallvolumesandstoreat-20°C.
      Applications
      ApplicationDetectp53withAnti-p53Antibody,aa211-220,clone240(MouseMonoclonalAntibody),thathasbeenshowntoworkinIP,WB&IHC.
      KeyApplications
      • Immunoprecipitation
      • WesternBlotting
      • Immunohistochemistry
      ApplicationsNotRecommended
      • Immunohistochemistry(Paraffin)
      ApplicationNotesDetectionofp53oncogeneprotein

      Detectionofmutantp53

      PrevalenceofdetectionusingCBL404

      -50%coloncarcinomasectionspositive(30samples)

      -70%lungcarcinomasectionspositive(50samples)

      -30%carcinomabreastsamplespositive(50samples)

      Normalandpre-malignanttissuesnegative

      Reactsonmethacarnfixedtissue

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenMurinep53-betagalactosidasefusionproteinexpressedinE.coli.
      Epitopeaa211-220
      ClonePAb240
      HostMouse
      SpecificityPAb240recognizesanepitopeofp53tumorsuppressorproteinbetweenaminoacids211and220(Gannon,1990;Legros,1994)inhuman,mouse,rat,hamster,monkey,cowandchicken.Assayingnativesamples(immunoprecipitation,ELISA)thePAb240detectsonlythemutantformsofp53(Gannon,1990;Said,1994).Inmethodsusingdenaturedsamples[Westernblotanalysis(Gannon,1990)andimmunohistochemistryoffrozentissuesections(Said,1994;Bartek,1991;Walker,1991)],thePAb240recognizesbothmutantanddenaturedwild-typep53.
      IsotypeIgG1
      SpeciesReactivity
      • Bovine
      • Chicken
      • Human
      • Hamster
      • Mouse
      • Monkey
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTumorproteinp53,anuclearprotein,playsanessentialroleintheregulationofcellcycle,specificallyinthetransitionfromG0toG1.Itisfoundinverylowlevelsinnormalcells,however,inavarietyoftransformedcelllines,itisexpressedinhighamounts,andbelievedtocontributetotransformationandmalignancy.p53isaDNA-bindingproteincontainingDNA-binding,oligomerizationandtranscriptionactivationdomains.Itispostulatedtobindasatetramertoap53-bindingsiteandactivateexpressionofdownstreamgenesthatinhibitgrowthand/orinvasion,andthusfunctionasatumorsuppressor.Mutantsofp53thatfrequentlyoccurinanumberofdifferenthumancancersfailtobindtheconsensusDNAbindingsite,andhencecausethelossoftumorsuppressoractivity.AlterationsoftheTP53geneoccurnotonlyassomaticmutationsinhumanmalignancies,butalsoasgermlinemutationsinsomecancer-pronefamilieswithLi-Fraumenisyndrome.
      GeneSymbol
      • TP53
      • TRP53
      • P53
      • p53
      • LFS1
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P04637#Actsasatumorsuppressorinmanytumortypes;inducesgrowtharrestorapoptosisdependingonthephysiologicalcircumstancesandcelltype.Involvedincellcycleregulationasatrans-activatorthatactstonegativelyregulatecelldivisionbycontrollingasetofgenesrequiredforthisprocess.Oneoftheactivatedgenesisaninhibitorofcyclin-dependentkinases.ApoptosisinductionseemstobemediatedeitherbystimulationofBAXandFASantigenexpression,orbyrepressionofBcl-2expression.
      COFACTOR:Binds1zincionpersubunit.
      SIZE:393aminoacids;43653Da
      SUBUNIT:InteractswithAXIN1.ProbablypartofacomplexconsistingofTP53,HIPK2andAXIN1(Bysimilarity).BindsDNAasahomotetramer.InteractswithhistoneacetyltransferasesEP300andmethyltransferasesHRMT1L2andCARM1,andrecruitsthemtopromoters.Invitro,theinteractionofTP53withcancer-associated/HPV(E6)viralproteinsleadstoubiquitinationanddegradationofTP53givingapossIBLemodelforcellgrowthregulation.Thiscomplexformationrequiresanadditionalfactor,E6-AP,whichstablyassociateswithTP53inthepresenceofE6.C-terminusinteractswithTAF1,whenTAF1ispartoftheTFIIDcomplex.InteractswithING4andthisinteractionmaybeindirect.FoundinacomplexwithCABLES1andTP73.InteractswithHIPK1,HIPK2,andP53DINP1.InteractswithWWOX.MayinteractswithHCVcoreprotein.InteractswithUSP7andSYVN1.InteractswithHSP90AB1(Bysimilarity).InteractswithBANP.
      SUBCELLULARLOCATION:Cytoplasm.Nucleus.Endoplasmicreticulum.Note=InteractionwithBANPpromotesnuclearlocalization.
      DOMAIN:SwissProt:P04637Thenuclearexportsignalactsasatranscriptionalrepressiondomain.
      PTM:Acetylated.AcetylationofLys-382byCREBBPenhancestranscriptionalactivity.DeacetylationofLys-382bySIRT1impairsitsABIlitytoinduceproapoptoticprogramandmodulatecellsenescence.&PhosphorylationonSerresiduesmediatestranscriptionalactivation.PhosphorylatedbyHIPK1(Bysimilarity).PhosphorylatedonThr-18byVRK1,whichmaypreventtheinteractionwithMDM2.PhosphorylatedonThr-55byTAF1,whichpromotesMDM2-mediateddegradation.PhosphorylatedonSer-46byHIPK2uponUVirrADIation.PhosphorylationonSer-46isrequiredforacetylationbyCREBBP.PhosphorylatedonSer-392followingUVbutnotgammairradiation.PhosphorylateduponDNAdamage,probablybyATMorATR.PhosphorylatedonSer-15uponultravioletirradiation;whichisenhancedbyinteractionwithBANP.&DephosphorylatedbyPP2A.SV40smallTantigeninhibitsthedephosphorylationbytheACformofPP2A.&MaybeO-glycosylatedintheC-terminalbasicregion.StudiedinEB-1cellline.&UbiquitinatedbySYVN1,whichleadstoproteasomaldegradation.
      DISEASE:SwissProt:P04637#TP53isfoundinincreasedamountsinawidevarietyoftransformedcells.TP53isfrequentlymutatedorinactivatedinabout60%ofcancers.&DefectsinTP53areinvolvedinesophagealsquamouscellcarcinoma(ESCC)[MIM:133239].ESCCisatumoroftheesophagus.&DefectsinTP53areacauseofLi-Fraumenisyndrome(LFS)[MIM:151623].LFSisanautosomaldominantfamilialcancersyndromethatinitsclassicformisdefinedbytheexistenceofbothaprobandwithasarcomaandtwootherfirst-degreerelativeswithacancerbyage45years.Inthesefamiliestheaffectedrelativesdevelopadiversesetofmalignanciesatunusuallyearlyages.ThespectrumofcancersinLFSincludesbreastcarcinomas,soft-tissuesarcomas,braintumors,osteosarcoma,leukemiaandadreno-corticalcarcinoma.OtherpossiblecomponenttumorsofLFSaremelanoma,gonadalcelltumorsandcarcinomasofthelung,pancreasandprostate.&DefectsinTP53maybeassociatedwithnasopharyngealcarcinoma[MIM:161550];alsoknownasnasopharyngealcancer.&DefectsinTP53arefoundinBarrettmetaplasia;alsoknownasBarrettesophagus.Itisaconditioninwhichthenormallystratifiedsquamousepitheliumoftheloweresophagusisreplacedbyametaplasticcolumnarepithelium.Theconditiondevelopsasacomplicationinapproximately10%ofpatientswithchronicgastroesophagealrefluxdiseaseandpredisposestothedevelopmentofesophagealadenocarcinoma.&DefectsinTP53areinvolvedinheadandnecksquamouscellcarcinomas(HNSCC)[MIM:275355].&DefectsinTP53areinvolvedinoralsquamouscellcarcinoma(OSCC).Cigarettesmokeisaprimemutagenicagentincanceroftheaerodigestivetract.&DefectsinTP53areacauseoflungcancer[MIM:211980].&DefectsinTP53areacauseofchoroidplexuspapilloma[MIM:260500].Choroidplexuspapillomaisaslow-growingbenigntumorofthechoroidplexusthatofteninvadestheleptomeninges.Inchildrenitisusuallyinalateralventriclebutinadultsitismoreofteninthefourthventricle.Hydrocephalusiscommon,eitherfromobstructionorfromtumorsecretionofcerebrospinalfluid.Ifitundergoesmalignanttransformationitiscalledachoroidplexuscarcinoma.Primarychoroidplexustumorsarerareandusuallyoccurinearlychildhood.&DefectsinTP53areacauseofoneformofhereditaryadrenocorticalcarcinoma(ADCC)[MIM:202300].ADCCisararechildhoodtumor,representingabout0.4%ofchildhoodtumors,withahighincidenceofassociatedtumors.ADCCoccurswithincreasedfrequencyinpatientswiththeBeckwith-Wiedemannsyndrome[MIM:130650]andisacomponenttumorinLi-Fraumenisyndrome[MIM:151623].
      SIMILARITY:Belongstothep53family.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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