Immunogen | HumanbreastcancercelllineZR-75-1. |
Epitope | 12-merepitope |
Clone | VU3C6 |
Host | Mouse |
Specificity | Theantibodyreactswiththeextracellular12-merGVTSAPDTRPAPepitopeoftheMUC-1tandemrepeat. |
Isotype | IgG1 |
SpeciesReactivity | |
AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Thisgeneisamemberofthemucinfamilyandencodesamembranebound,glycosylatedphosphoprotein.Theproteinisanchoredtotheapicalsurfaceofmanyepitheliabyatransmembranedomain,withthedegreeofglycosylationvaryingwithcelltype.Italsoincludesa20aavariablenumbertandemrepeat(VNTR)domain,withthenumberofrepeatsvaryingfrom20to120indifferentindividuals.Theproteinservesaprotectivefunctionbybindingtopathogensandalsofunctionsinacellsignalingcapacity.Overexpression,aberrantintracellularlocalization,andchangesinglycosylationofthisproteinhavebeenassociatedwithcarcinomas.Multiplealternativelysplicedtranscriptvariantsthatencodedifferentisoformsofthisgenehavebeenreported,butthefull-lengthnatureofonlysomehasbeendetermined. |
GeneSymbol | - MUC1
- MUC-1/X
- PEMT
- MUC-1/SEC
- PUM
- MUC1/ZD
- MUC-1
- Episialin
- episialin
- EMA
- PEM
- H23AG
- MAM6
- CD227
|
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P15941#Isoform7behavesasareceptorandbindsthesecretedisoform5.Thebindinginducesthephosphorylationoftheisoform7,alterscellularmorphologyandinitiatescellsignaling.CanbindtoGRB2adapterprotein. SIZE:1255aminoacids;122072Da SUBUNIT:Thecleavedform(onlytheisoform1)ofMUC1formsatightheterodimerwiththereleasedC-terminalpeptide(whichisfirstsecretedtobeextracellular). SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein.&Isoform5:Secreted.&Isoform9:Secreted. TISSUESPECIFICITY:ExpressedonductalepithelialcellsandonactivatedT-cells.Aberrantlyglycosylatedformsexpressedinhumanepithelialtumors,suchasbreastorovariancancerandalsoinnon-epithelialtumorcells.Isoform7expressedonlyintumoralcells. PTM:Highlyglycosylated(N-andO-linkedcarbohydratesandsialicacid).Inthe20aminoacidtandemrepeatpositions5(Ser),6(Thr),14(Thr),15(Ser)and19(Thr)areO-glycosylated(GalNAc).TheaveragedensityofO-glycosylatedsiteswithinrepeatpeptidesvarieswithcelldifferentiationfromabout50%inlactation-associatedMUC1toover90%inavarietyofbreastcancercells.&Isoform1andisoform7undergoestransphosphorylationonserineandtyrosineresidues. SIMILARITY:SwissProt:P15941##Contains1SEAdomain. |