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Millipore/APT165 | Caspase 3 Colorimetric Activity Assay Kit, DEVD/APT165/100 assays
  • Millipore/APT165 | Caspase 3 Colorimetric Activity Assay Kit, DEVD/APT165/100 assays

Millipore/APT165 | Caspase 3 Colorimetric Activity Assay Kit, DEVD/APT165/100 assays

價格: ¥6108.00 市場價: 10180.00

貨號: APT165
品牌: Millipore
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    • Description
      CatalogueNumberAPT165
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionCaspase3ColorimetricActivityAssayKit,DEVD
      OverviewActivationofICE-familyproteases/caspasesinitiatesapoptosisinmammaliancells.Chemicon"sCaspase-3ColorimetricActivityAssayKitsprovideasimpleandconvenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceDEVD.Theassayisbasedonspectophotometricdetectionofthechromophorep-nitroaniline(pNA)aftercleavagefromthelabeledsubstrateDEVD-pNA.ThefreepNAcanbequantifiedusingaspectrophotometeroramicrotiterplatereaderat405nm.ComparisonoftheabsorbanceofpNAfromanapoptoticsamplewithanuninducedcontrolallowsdeterminationofthefoldincreaseincaspase-3activity.
      AlternateNames
      • CPP32
      MaterialsRequiredbutNotDelivered·Microcentrifugeand1.5mLMicrocentrifugetubes

      ·37°CWaterbathorIncubator

      ·SpectrophotometerorMicroplateReader

      ·96wellMicrotiterPlate
      ProductInformation
      Components
      • 5XCellLysisBuffer(PartNo.90065):5mL
      • 5XAssayBuffer(PartNo.90066):10mL
      • Caspase-3Substrate(Ac-DEVD-pNA)(PartNo.90080):1mLof3mg/mLsolution
      • Caspase-3Inhibitor(Ac-DEVD-CHO)(PartNo.90067):50μLof100μM(0.05mg/mL)inDMSO
      • pNAStandard(PartNo.90085):250μLof10mMinDMSO
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditionsStorekitmaterialsat-20°Cuptotheirexpirationdate.

      SpecialPrecautions:

      ·Afterthawingreagents,useimmediatelyoraliquotandfreezeat-20oCforlongerstorage.Avoidrepeatedfreeze/thawcycles.

      ·TheCaspase-3substrateandpNAstandardareespeciallylightsensitive.Maintainthesereagentsinamberorcoveredcontainers.
      Applications
      ApplicationCaspase-3ColorimetricActivityAssayKitsprovideasimple&convenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceDEVD.
      KeyApplications
      • ActivityAssay
      BIOLOGicalInformation
      SpeciesReactivity
      • Mammals
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisproteincleavesandactivatescaspases6,7and9,andtheproteinitselfisprocessedbycaspases8,9and10.Itisthepredominantcaspaseinvolvedinthecleavageofamyloid-beta4Aprecursorprotein,whichisassociatedwithneuronaldeathinAlzheimer"sdisease.Alternativesplicingofthisgeneresultsintwotranscriptvariantsthatencodethesameprotein.
      GeneSymbol
      • CASP3
      • apopain
      • procaspase3
      • CPP-32
      • Apopain
      • Yama
      • CASP-3
      • CPP32B
      • SCA-1
      • CPP32
      • EC3.4.22.56[Contains:Caspase-3subunitp17
      • Caspase-3subunitp12].
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P42574#InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Attheonsetofapoptosisitproteolyticallycleavespoly(ADP-ribose)polymerase(PARP)ata"216-Asp--Gly-217"bond.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs)betweenthebasichelix-loop-helixleucinezipperdomainandthemembraneattachmentdomain.Cleavesandactivatescaspase-6,-7and-9.Involvedinthecleavageofhuntingtin.
      SIZE:277aminoacids;31608Da
      SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya17kDa(p17)anda12kDa(p12)subunit.
      SUBCELLULARLOCATION:Cytoplasm.
      TISSUESPECIFICITY:Highlyexpressedinlung,spleen,heart,liverandkidney.Moderatelevelsinbrainandskeletalmuscle,andlowintestis.Alsofoundinmanycelllines,highestexpressionincellsoftheimmunesystem.
      PTM:CleavagebygranzymeB,caspase-6,caspase-8andcaspase-10generatesthetwoactivesubunits.Additionalprocessingofthepropeptidesislikelyduetotheautocatalyticactivityoftheactivatedprotease.Activeheterodimersbetweenthesmallsubunitofcaspase-7proteaseandthelargesubunitofcaspase-3alsooccurandviceversa.
      SIMILARITY:SwissProt:P42574##BelongstothepeptidaseC14family.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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