當前位置 : Millipore >>> Millipore/APT116 | Apo-BRDU IHC Kit/APT116/50 assays
Millipore/APT116 | Apo-BRDU IHC Kit/APT116/50 assays
  • Millipore/APT116 | Apo-BRDU IHC Kit/APT116/50 assays

Millipore/APT116 | Apo-BRDU IHC Kit/APT116/50 assays

價格: 試用 市場價: 0.00

貨號: APT116
品牌: Millipore
規(guī)格
數(shù)量
庫存(0)
特別 提示
代購產(chǎn)品:無質(zhì)量問題不接受退換貨,下單前請仔細核對信息。
下單后請及時聯(lián)系客服核對商品價格,訂單生效后再付款。
資深產(chǎn)品顧問
咨詢顧問

全國免費服務熱線

4000-520-616


  • 自營商城 一站式服務
  • 廠家直采 剔除溢價
  • 品質(zhì)甄選 正品保證
  • 嚴控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說明
  • 常見問題
    • Description
      CatalogueNumberAPT116
      BrandFamilyChemicon®
      TradeName
      • Apo-BrdU
      • Chemicon
      DescriptionApo-BRDUIHCKit
      OverviewTheCHEMICONAPO-BRDU-IHC?KitisatwocolorstainingmethodforlabelingDNAbreaksandtotalcellularDNAtodetectapoptoticcellsbyimmunohistochemistry(Lietal.1995).Thekitcontainstheinstructionsandreagentsrequiredformeasuringapoptosisincellsincluding:positiveandnegativecontrolslidesforassessingreagentperformance;reactionandblockingbuffersforprocessingindividualstepsintheassay;terminaldeoxynucleotidyltranferaseenzyme(TdT),bromodeoxyuridinetriphosphate(Br-dUTP),biotin-labeledanti-BrdUantibodyforlabelingDNAstrandbreaks,HRP-streptavidinconjugate,DAN,H2O2/Ureatabletsforcolorgeneration,andmethylgreensolutionforcounterstainingthecells.
      MaterialsRequiredbutNotDelivered1.Xylene-100%

      2.Ethanol-100%,90%,80%,70%v/vwithdH2O

      3.Methanol-100%

      4.HydrogenPeroxide-30%

      5.PBSorTris-bufferedsaline(1XTBS,20mMTrispH7.6,140mMNaCl)

      6.10mMTris,pH8.0

      7.1mMMgSO4in1XTBS(foruseingeneratingpositivecontrol)

      8.DNaseI(foruseingeneratingpositivecontrol)

      9.1-4%FormaldehydeinPBS,pH7.4

      10.DistilledWater

      11.Coplinjarswithslideholders

      12.Beakerforrinsingslides

      13.Slidehumidifyingchamber

      14.Glasscoverslips

      15.Mountingmedia

      16.Microscope

      17.Micro-pipettorwithdisposabletips

      18.Microcentrifugetubes

      19.Parafilm?(RegisteredtrademarkofAmericanNationalCanCompany)

      20.Absorbentwipes

      21.Crushedice
      ProductInformation
      Components
      • Positive/NegativeControlSlides,Naturalbox
      • Note:2slides,eachcontainingamixturepositiveandnegativecontrolcells.
      • BlockingBuffer,Whitecap
      • H2O2/UreaTablets,Ambervial
      • ProteinaseK,Pinkcap
      • DABTablets,Ambervial
      • TdTEnzyme,Yellowcap
      • Br-Dutp,Violetcap
      • 200XStreptavidin-HRPConjugate,Blackcap
      • 5XReactionBuffer,Greencap
      • Biotin-labeledBrdUmAb,Orangecap
      • MethylGreensolution,Naturalcap,
      • TheAPO-BRDU-IHC?Kitisshippedinonecontainerandshouldbestoredat-20°Cuponarrival.
      • Note:Afterthekitisdefrostedbeforefirstuse,boththe5XReactionBufferandtheBiotin-labeledanti-BrdUantibodyshouldbestoredat2-8°C.Also,theMethylGreensolutionshouldbestoredatroomtemperature.DONOTRE-FREEZEanyofthesecomponentsoncethawed.
      • Reagentbottleshavecolorcodedcapstoaidintheiridentification.Sufficientreagentsareprovidedtoprocess50slidesincluding2positive/negativecontrolslides.Thecontrolcellsarederivedfromahumanpromyelociticleukemiacellline(HL60)andincubatedwith0.5mg/mLactinomycinDfor19hourstoinduceapoptosis.
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditionsPrecautionsandWarnings:

      1.ThecomponentsofthiskitareforResearchUseonlyandarenotintendedfordiagnosticprocedures.

      2.Afterinitialdefrosting,storethe5XReactionBufferandBiotin-labeledanti-BrdUantibodyat2-8oC,andtheMethylGreensolutionatroomtemperature.DONOTRE-FREEZEanyofthesecomponentsoncethawed.

      3.TdTEnzymewillnotfreezeat-20oC,becauseitisin50%(v/v)glycerolsolution.

      4.The5XReactionbufferandBr-dUTPsolutioncontaincacodylicacidasabuffer.TheBiotin-labeledantibodycontains0.05%sodiumazideasapreservative.TheDABtabletscontaindiaminobenzidine.Thesematerialsareharmfulifswallowed.Avoidskincontactwithanyofthesecomponents;washimmediatelywithwater.

      5.Uponwarmingthecomponents,centrifugethetubesfor30secondstoforcealltheliquidtothebottomofthetube.

      6.Gloves,labcoatandprotectiveeyewearshouldbewornwhilestaining.
      Applications
      ApplicationTheAPO-BRDU-IHCKitisatwocolorstainingmethodforlabelingDNAbreaks&totalcellularDNAtodetectapoptoticcellsbyIHC.
      KeyApplications
      • Immunohistochemistry
      BIOLOGicalInformation
      SpeciesReactivity
      • All
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    螞蟻淘生物188,秉承螞蟻淘一貫的嚴謹態(tài)度,由螞蟻淘公司專業(yè)人員負責品控、采購、物流、銷售、售后,保障正品優(yōu)質(zhì)。以“快速好省,為科研提供好產(chǎn)品、好價格”為理念,直接鏈接原廠家,從全國各地原制造商嚴格挑選188款科研精品,剔除品牌溢價,188生物新電商,把好的產(chǎn)品帶給科研!? 力求給你最優(yōu)質(zhì)的商品。
  • Q:生物188產(chǎn)品正品保障嗎?
    A:生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗,在業(yè)界享有良好的口碑;自營商城,直接從廠家采購, 自己的團隊負責國際物流和清關(guān),中間沒有第三方,所有流程嚴格把控,100%保證正品,假一罰十。

    Q:下單后可以修改訂單嗎?
    A:下單后的商品付款之前可以修改;訂單付款成功,需要聯(lián)系我們客服進行修改;客服電話:4000-520-616

    Q:可以開發(fā)票嗎?
    A:本網(wǎng)站所售商品都是正規(guī)清關(guān),均開具16%正規(guī)發(fā)票,發(fā)票金額含配送費金額,另有說明的除外。

    Q:商品幾天可以發(fā)貨?
    A:生物188商品,全部現(xiàn)貨銷售,付款后即可發(fā)貨,一般一周內(nèi)送達!

    Q:如何聯(lián)系商家?
    A:有任何問題夠可以電話咨詢我們,全國24小時免費服務熱線:4000-520-616 或聯(lián)系我們的在線客服QQ:1570468124

    Q:收到的商品少了/發(fā)錯了怎么辦?
    A:同個訂單購買多個商品可能會分為一個以上包裹發(fā)出,可能不會同時送達,建議查看訂單詳情是否是 部分發(fā)貨狀態(tài);如未收到,可聯(lián)系在線客服或者致電4000-520-616。

    Q:退換貨/維修需要多長時間?
    A:一般情況下,退貨處理周期為客戶收到產(chǎn)品一個月內(nèi)(以快遞公司顯示簽收時間為準),包裝規(guī)格、 數(shù)量、品種不符,外觀毀損、短缺或缺陷,請在收到貨24小時內(nèi)申請退換貨;特殊商品以合同條款為準。

何為188

極簡而嚴謹,我們僅銷售188款生物醫(yī)學科研用品,款款都是爆款;因為少所以聚焦,聚焦甄選每一款產(chǎn)品,聚焦服務每一位客戶!

關(guān)注我們 :

點擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號

獲取最新優(yōu)惠活動通知
  • 品質(zhì)甄選,正品保證

  • 自營電商,廠家直采

  • 極簡主義,188精品