Description
SUPERase?In?RNaseInhibitorisaprotein-basedinhibitorofnonhumanoriginthatnoncovalentlybindsandinhibitsthemostcommonandtroublesomeRNases,includingRNaseA,B,C,1,andT1.FeaturesofSUPERase?In?RNaseInhibitor:
?CompletelyremovesRNasecontaminationfromglassandplasticsurfaces
?ExcelsatremovinghighlevelsofRNasecontaminationwheresimilarproductsfail
?Proveneffectiveatremovinghighconcentrationsofdried-onRNaseA
?Idealforcleaningworksurfaces,pipettors,andequipmentthatmustbeRNase-free
SUPERase?In?RNaseInhibitorcanbeusedinanyapplicationwhereRNasecontaminationcouldbeproblematic.BecauseitinhibitsabroaderrangeofRNasesthantrADItionalRNaseinhibitors,SUPERase?In?isthemosteffectiveRNaseinhibitoravailable,providingahigherlevelofprotectionagainstdegradation.
SUPERase?In?doesnotinterferewithotherenzymessuchasRNApolymerases,reversetranscriptase,or Taq DNApolymerase.Additionally,SUPERase?In?isactiveupto65°CandoverapHrangeof5.5to8.5.
Ifyouareplannningtousethisproductwithstandardantibodypurificationmethods,pleasecontactourtechnicalsupporttodiscussexperimentaldesign.
Unitdefinition
SUPERase?In?RNaseInhibitorat1U/μLwillblockthedegradationof0.1μg/μLlabeledRNAby2.5pg/μLofRNaseA,2.5pg/μLofRNaseI,and0.0075U/μLofRNaseT1,for4hoursat37°C,in20mMTris-HCl,pH7.5,50mMNaCl,1mMEDTA.AnalysisisbydenaturingPAGE.SUPERase
?Iniscurrentlytheonlyribonucleaseinhibitorforwhichtheunitactivityisdefinedbysuchafunctionalassay.