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Millipore/AB9927 | Anti-phospho-Akt1 (Tyr326) Antibody/AB9927/100 µg
  • Millipore/AB9927 | Anti-phospho-Akt1 (Tyr326) Antibody/AB9927/100 µg

Millipore/AB9927 | Anti-phospho-Akt1 (Tyr326) Antibody/AB9927/100 µg

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貨號(hào): AB9927
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    • Description
      CatalogueNumberAB9927
      Replaces09-288
      DescriptionAnti-phospho-Akt1(Tyr326)Antibody
      AlternateNames
      • RAC-alphaserine/threonine-proteinkinase
      • ProteinkinaseB
      • PKB
      • Proto-oncogenec-Akt
      • RAC-PK-alpha
      BackgroundInformationTheserine/threoninekinaseAktfamilycontainsseveralmembers,includingAkt1(alsodesignatedPKBorRacPK),Akt2(alsodesignatedPKB-βorRacPK-β)andAkt3(alsodesignatedPKB-γorthyomaviralproto-oncogene3),whichexhibitsequencehomologywiththeproteinkinaseAandCfamiliesandareencodedbythec-Aktproto-oncogene.AllmembersoftheAktfamilyhaveaPleckstrinhomologydomain.Akt1andAkt2areactivatedbyPDGFstimulation.ThisactivationisdependentonPDGFR-βtyrosineresidues740and751,whichbindthe85kDasubunitofthephosphatidylinositol3-kinase(PI3-kinase)complex.TheactivationofAkt1andAkt2isinhibitedbythePIkinaseinhibitorwortmannin.Takentogether,thisdatastronglysuggeststhattheproteinsignalsdownstreamofthePIkinases.
      ProductInformation
      FormatAffinityPurified
      Control
      • EGFtreatedanduntreatedHeLacelllysates
      PresentationPurifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
      Applications
      ApplicationDetectphospho-Akt1(Tyr326)usingthisAnti-Akt1AntibodyvalidatedforuseinWB.
      KeyApplications
      • WesternBlotting
      BIOLOGicalInformation
      ImmunogenKLH-conjugatedlinearpeptidecorrespondingtohumanAkt1phosphorylatedatTyr326.
      EpitopePhosphorylatedTyr326
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      SpecificityThisantibodyrecognizesAkt1phosphorylatedatTyr326.
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      • Canine
      • Xenopus
      SpeciesReactivityNoteDemonstratedtoreactwithHuman.PredictedtoreactwithMouse,Rat,Canine,andXenopusbasedon100%sequencehomology.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheserine-threonineproteinkinaseencodedbytheAKT1geneiscatalyticallyinactiveinserum-starvedprimaryandimmortalizedfibroblasts.AKT1andtherelatedAKT2areactivatedbyplatelet-derivedgrowthfactor.Theactivationisrapidandspecific,anditisabrogatedbymutationsinthepleckstrinhomologydomainofAKT1.Itwasshownthattheactivationoccursthroughphosphatidylinositol3-kinase.InthedevelopingnervoussystemAKTisacriticalmediatorofgrowthfactor-inducedneuronalsurvival.Survivalfactorscansuppressapoptosisinatranscription-independentmannerbyactivatingtheserine/threoninekinaseAKT1,whichthenphosphorylatesandinactivatescomponentsoftheapoptoticmachinery.Multiplealternativelysplicedtranscriptvariantshavebeenfoundforthisgene.[providedbyRefSeq].
      GeneSymbol
      • AKT1
      • PKB
      • RAC
      Modifications
      • Phosphorylation
      PurificationMethodAffinityPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:PlaysaroleasakeymodulatoroftheAKT-mTORsignalingpathwaycontrollingthetempooftheprocessofnewbornneuronsintegrationduringadultneurogenesis,includingcorrectneuronpositioning,dendriticdevelopmentandsynapseformation(Bysimilarity).Generalproteinkinasecapableofphosphorylatingseveralknownproteins.PhosphorylatesTBC1D4.Signalsdownstreamofphosphatidylinositol3-kinase(PI3K)tomediatetheeffectsofvariousgrowthfactorssuchasplatelet-derivedgrowthfactor(PDGF),epidermalgrowthfactor(EGF),insulinandinsulin-likegrowthfactorI(IGF-I).Playsaroleinglucosetransportbymediatinginsulin-inducedtranslocationoftheGLUT4glucosetransportertothecellsurface.MediatestheantiapoptoticeffectsofIGF-I.Mediatesinsulin-stimulatedproteinsynthesisbyphosphorylatingTSC2at"Ser-939"and"Thr-1462",therebyactivatingmTORC1signalingandleADIngtobothphosphorylationof4E-BP1andinactivationofRPS6KB1.Promotesglycogensynthesisbymediatingtheinsulin-inducedactivationofglycogensynthase.TheactivatedformcansuppressFoxOgenetranscriptionandpromotecellcycleprogression.EssentialfortheSPATA13-mediatedregulationofcellmigrationandadhesionassemblyanddisassembly.Ref.6Ref.10Ref.14Ref.15Ref.17Ref.19CATALYTICACTIVITY:ATP+aprotein=ADP+aphosphoprotein.

      ENZYMEREGULATION:Threespecificsites,oneinthekinasedomain(Thr-308)andthetwootheronesintheC-terminalregulatoryregion(Ser-473andTyr-474),needtobephosphorylatedforitsfullactivation.

      SUBUNITSTRUCTURE:InteractswithAGAP2(isoform2,PIKE-A),theinteractionrequiresguaninenucleotidesandstimulatesthekinaseactivity.Interacts(viatheC-terminus)withCCDC88A(viaitsC-terminus)andTHEM4(viaitsC-terminus).InteractswithAKTIP.Interacts(viaPHdomain)withMTCP1,TCL1AANDTCL1B.InteractswithTRAF6.InteractswithGRB10;theinteractionleadstoGRB10phosphorylationthuspromotingYWHAEbinding.InteractswithRARA;theinteractionphosphorylatesRARAandrepressesitstransactivationactivity.InteractswithTNK2.

      SUBCELLULARLOCATION:Cytoplasm.Nucleus.Cellmembrane.Note:Nucleusafteractivationbyintegrin-linkedproteinkinase1(ILK1).NucleartranslocationisenhancedbyinteractionwithTCL1A.PhosphorylationonTyr-176byTNK2resultsinitslocalizationtothecellmembranewhereitistargetedforfurtherphosphorylationsonThr-308andSer-473leadingtoitsactivationandtheactivatedformtranslocatestothenucleus.

      TISSUESPECIFICTY:Expressedinallhumancelltypessofaranalyzed.TheTyr-176phosphorylatedformshowsasignificantincreaseinexpressioninbreastcancersduringtheprogressivestagesi.e.normaltohyperplasia(ADH),ductalcarcinomainsitu(DCIS),invasiveductalcarcinoma(IDC)andlymphnodemetastatic(LNMM)stages.DOMAIN:BindingofthePHdomaintothephosphatidylinositol3-kinasealpha(PI3K)resultsinitstargetingtotheplasmamembrane.ThePHdomainmediatesinteractionwithTNK2andTyr-176isalsoessentialforthisinteraction.

      TheAGC-kinaseC-terminalmediatesinteractionwithTHEM4.

      PTM:PhosphorylationonThr-308,Ser-473andTyr-474isrequiredforfullactivity.ActivatedTNK2phosphorylatesitonTyr-176resultinginitsbindingtotheanionicplasmamembranephospholipidPA.Thisphosphorylatedformlocalizestothecellmembrane,whereitistargetedbyPDPK1andPDPK2forfurtherphosphorylationsonThr-308andSer-473leadingtoitsactivation.Ser-473phosphorylationbymTORC2favorsThr-308phosphorylationbyPDPK1.Ser-473phosphorylationisenhancedbyinteractionwithAGAP2isoform2(PIKE-A).Ser-473phosphorylationisenhancedinfocalcorticaldysplasiaswithTaylor-typeballooncells.

      Ubiquitinated;undergoesboth"Lys-48"-and"Lys-63"-linkedpolyubiquitination.TRAF6-induced"Lys-63"-linkedAKT1ubiquitinationiscriticalforphosphorylationandactivation.Whenubiquitinated,ittranslocatestotheplasmamembrane,whereitbecomesphosphorylated.Whenfullyphosphorylatedandtranslocatedintothenucleus,undergoes"Lys-48"-polyubiquitinationcatalyzedbyTTC3,leadingtoitsdegradationbytheproteasome.Ref.10Ref.14Ref.17Ref.34Ref.8Ref.20Ref.9Ref.16Ref.21Ref.26Ref.27INVOLVEMENTINDISEASE:DefectsinAKT1areacauseofsusceptibilitytobreastcancer(BC)[MIM:114480].Acommonmalignancyoriginatingfrombreastepithelialtissue.Breastneoplasmscanbedistinguishedbytheirhistologicpattern.Invasiveductalcarcinomaisbyfarthemostcommontype.Breastcancerisetiologicallyandgeneticallyheterogeneous.Importantgeneticfactorshavebeenindicatedbyfamilialoccurrenceandbilateralinvolvement.Mutationsatmorethanonelocuscanbeinvolvedindifferentfamiliesoreveninthesamecase.

      DefectsinAKT1areassociatedwithcolorectalcancer(CRC)[MIM:114500].

      DefectsinAKT1areassociatedwithsusceptibilitytoovariancancer[MIM:604370];alsocalledsusceptibilitytofamilialbreast-ovariancancertype1(BROVCA1).

      SEQUENCESIMILARITIES:Belongstotheproteinkinasesuperfamily.AGCSer/Thrproteinkinasefamily.RACsubfamily.

      Contains1AGC-kinaseC-terminaldomain.

      Contains1PHdomain.

      Contains1proteinkinasedomain.
      MolecularWeight~56kDaobserved
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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