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Millipore/AB9872 | Anti-TrkB Antibody/AB9872/50 µL
  • Millipore/AB9872 | Anti-TrkB Antibody/AB9872/50 µL

Millipore/AB9872 | Anti-TrkB Antibody/AB9872/50 µL

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貨號: AB9872
品牌: Millipore
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    • Description
      CatalogueNumberAB9872
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-TrkBAntibody
      AlternateNames
      • TyrosinekinasereceptorB
      BackgroundInformationThemammalianTrkfamilyofreceptortyrosinekinasesconsistsofthreeprincipletransmembraneproteins.Thereceptorscanbeactivatedbyoneormoreoffourneurotrophins-NGF,BDNF,NT3andNT4/5.TheTrkBfamilyoftransmembraneproteinsservesasreceptorsforBDNFandNT-4/5andtheseneurotrophinsregulateBIOLOGicalresponsessuchascellsurvivial,proliferationanddifferentiationinnormalandneoplasticneuronalcells.
      ProductInformation
      FormatSerum
      PresentationLyophilized.Reconstitutewith50μLofsteriledistilledwater.Centrifugetoremoveanyinsolublematerial.Containsnopreservative.
      StorageandShippingInformation
      StorageConditionsMaintainlyophilizedmaterialfrozenat-20°Cto-70°Cforupto12monthsafterdateofreceipt.Afterreconstitutionmaintainat-20°Cinundilutedaliquotsforupto6months.Avoidrepeatedfreeze/thawcycles.Glycerol(1:1,ACSorbettergrade)canbeaddedforadditionalstABIlity.
      Applications
      ApplicationAnti-TrkBAntibodyisanantibodyagainstTrkBforuseinIH.
      KeyApplications
      • Immunohistochemistry
      ApplicationNotesImmunohistochemistry:1:3,000.Theantibodyhasbeenusedsuccessfullyonratdorsalrootganglionandtrigeminalgangliaintheratspinalcord(cryostatfree-floatingsections).Suggestedfixativeis4%paraformaldehyde.

      NotsuitableforWesternblot.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BiologicalInformation
      ImmunogenExtracellulardomainoftherecombinantmousereceptorexpressedinmammaliancells.
      HostRabbit
      SpecificitytrkB[BDNF/NT-4/5growthfactorreceptor;tyrosinekinasereceptorB].TheimmunogensequencewaschosentoavoidcrossreactivitywithtrkAortrkC.
      SpeciesReactivity
      • Mouse
      • Rat
      SpeciesReactivityNoteOtherspecieshavenotyetbeentested.Itisexpectedtoreactwithawiderangeofmammalianspecies,basedonthehighsequencesimilarity.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesamemberoftheneurotrophictyrosinereceptorkinase(NTRK)family.Thiskinaseisamembrane-boundreceptorthat,uponneurotrophinbinding,phosphorylatesitselfandmembersoftheMAPKpathway.Signallingthroughthiskinaseleadstocelldifferentiation.Mutationsinthisgenehavebeenassociatedwithobesityandmooddisorders.Alternatetranscriptionalsplicevariantsencodingdifferentisoformshavebeenfoundforthisgene.
      GeneSymbol
      • NTRK2
      • Trk-B
      • TRKB
      • GP145-TrkB
      • EC2.7.10.1
      PurificationMethodSerum
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q16620#Receptorforbrain-derivedneurotrophicfactor(BDNF),neurotrophin-3andneurotrophin-4/5butnotnervegrowthfactor(NGF).Involvedinthedevelopmentand/ormaintenanceofthenervoussystem.Thisisatyrosine-proteinkinasereceptor.KnownsubstratesfortheTRKreceptorsareSHC1,PI-3kinase,andPLC-gamma-1.
      SIZE:822aminoacids;91999Da
      SUBUNIT:Existsinadynamicequilibriumbetweenmonomeric(lowaffinity)anddimeric(highaffinity)structures.BindsSH2B2.InteractswithSQSTM1(Bysimilarity).
      SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
      TISSUESPECIFICITY:IsoformTrkBiswidelyexpressed,mainlyinthenervoustissue.IntheCNS,expressionisobservedinthecerebralcortex,hippocampus,thalamus,choroidplexus,granularlayerofthecerebellum,brainstem,andspinalcord.Intheperipheralnervoussystem,itisexpressedinmanycranialganglia,theophtalmicnerve,thevestibularsystem,multiplefacialstructures,thesuBMAxillaryglands,anddorsalrootganglia.IsoformTrkB-T1isexpressedinmultipletissues,mainlyinbrain,pancreas,kidneyandheart.IsoformTrkB-T-Shcispredominantlyexpressedinbrain.
      PTM:Ligand-mediatedauto-phosphorylation.
      SIMILARITY:SwissProt:Q16620##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.Insulinreceptorsubfamily.&Contains2Ig-likeC2-type(immunoglobulin-like)domains.&Contains2LRR(leucine-rich)repeats.&Contains1proteinkinasedomain.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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