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Millipore/AB9734 | Anti-14-3-3 ε Antibody, NT/AB9734/100 µL
  • Millipore/AB9734 | Anti-14-3-3 ε Antibody, NT/AB9734/100 µL

Millipore/AB9734 | Anti-14-3-3 ε Antibody, NT/AB9734/100 µL

價(jià)格: ¥4068.00 市場(chǎng)價(jià): 6780.00

貨號(hào): AB9734
品牌: Millipore
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    • Description
      CatalogueNumberAB9734
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-14-3-3εAntibody,NT
      BackgroundInformationMammalsexpresssevendistinct14-3-3isoforms(gamma,epsilon,beta,zeta,sigma,theta,tau)thatformmultiplehomo-andhetero-dimmers,thusrendering14-3-3asakeymultifunctionalregulatorymolecule.14-3-3isomershavebeenimplicatedinseveralneurologicaldisorders:forexampleisoformsgamma,epsilonandbetaareexpressedinthecerebrospinalfluidofCreutzfeldt-Jakobpatients,butnotpatientssufferingfromotherneurologicalconditionssuchasAlzheimer"s.

      The14-3-3epsilon,C-terminalantibodyisoneofapanelofantibodiesthatweregeneratedagainst14-3-3isoformsandhavedemonstratedtheirpotentialforuseasanELISA-baseddiagnostictool.TheseantibodieswillallowtheresearchtoinvestigatetheroleofthisproteininkeycellularprocessesandneurologicaldisorderssuchasCreutzfeldt-Jakob,Alzheimer"sandParkinson"sDisease.
      ProductInformation
      FormatSerum
      PresentationLiquid.
      StorageandShippingInformation
      StorageConditionsMaintainat-20°Cinundilutedaliquotsforupto6monthsafterdateofreceipt.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationThisAnti-14-3-3εAntibody,N-terminalisvalidatedforuseinWB,IHforthedetectionof14-3-3ε.
      KeyApplications
      • WesternBlotting
      • Immunohistochemistry
      ApplicationNotesWesternblot:1:3,000usingECL.Immunohistochemistry:1:200-1:400onmousebraintissuewhichwastreatedbyhydratedautoclavingat121°Cfor15minutespriortoimmunolabeling.Endogenousperoxidasewasblockedwithhydrogenperoxideinmethanolandnon-specificlabelingwithnormalgoatserumat1:20for5minutes.ELISAOptimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenSyntheticpeptidefromtheN-terminalareaofsheep14-3-3epsilon.
      HostRabbit
      Specificity14-3-3epsilon,N-terminal.ByWesternblottheantibodyrecognizesthe~33kDaprotein.
      SpeciesReactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Sheep
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneproductbelongstothe14-3-3familyofproteinswhichmediatesignaltransductionbybindingtophosphoserine-containingproteins.Thishighlyconservedproteinfamilyisfoundinbothplantsandmammals,andthisproteinis100%identicaltothemouseortholog.ItinteractswithCDC25phosphatases,RAF1andIRS1proteins,suggestingitsroleindiversebiochemicalactivitiesrelatedtosignaltransduction,suchascelldivisionandregulationofinsulinsensitivity.Ithasalsobeenimplicatedinthepathogenesisofsmallcelllungcancer.
      GeneSymbol
      • YWHAE
      • KCIP-1
      • MDS
      • 14-3-3E
      • MDCR
      • FLJ45465
      PurificationMethodSerum
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P62258#Adapterproteinimplicatedintheregulationofalargespectrumofbothgeneralandspecializedsignalingpathway.Bindstoalargenumberofpartners,usuallybyrecognitionofaphosphoserineorphosphothreoninemotif.Bindinggenerallyresultsinthemodulationoftheactivityofthebindingpartner.
      SIZE:255aminoacids;29174Da
      SUBUNIT:Homodimer.InteractswithNDEL1(Bysimilarity).InteractswithHCVcoreprotein.
      SUBCELLULARLOCATION:Cytoplasm(Bysimilarity).Melanosome.Note=IdentifiedbymassspectrometryinmelanosomefractionsfromstageItostageIV.
      SIMILARITY:SwissProt:P62258##Belongstothe14-3-3family.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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