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Millipore/AB9568 | Anti-Neurofilament L Antibody/AB9568/50 µL
  • Millipore/AB9568 | Anti-Neurofilament L Antibody/AB9568/50 µL

Millipore/AB9568 | Anti-Neurofilament L Antibody/AB9568/50 µL

價格: ¥3072.00 市場價: 5120.00

貨號: AB9568
品牌: Millipore
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    • Description
      CatalogueNumberAB9568
      Replaces04-1112
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-NeurofilamentLAntibody
      BackgroundInformationNeurofilamentsareatypeofintermediatefilamentthatserveasmajorelementsoftheCytoskeletonsupportingtheaxoncytoplasm.Theyarethemostabundantfibrillarcomponentsoftheaxon,beingonaverage3-10timesmorefrequentthanaxonalmicrotubules.Neurofilaments(10nmindia.)arebuiltfromthreeintertwinedprotofibrilswhicharethemselvescomposedoftwotetramericprotofilamentcomplexesofmonomericproteins.Theneurofilamenttripletproteins(68/70,160,and200kDa)occurinboththecentralandperipheralnervoussystemandareusuallyneuronspecific.The68/70kDaNF-Lproteincanself-assembleintoafilamentousstructure,howeverthe160kDaNF-Mand200kDaNF-Hproteinsrequirethepresenceofthe68/70kDaNF-Lproteintoco-assemble.Neuromas,ganglioneuromas,gangliogliomas,ganglioneuroblastomasandneuroblastomasstainpositivelyforneurofilaments.Althoughtypicallyrestrictedtoneurons,neurofilamentshavebeendetectedinparagangliomasandadrenalandextra-adrenalpheochromocytomas.Carcinoids,neuroendocrinecarcinomasoftheskin,andoatcellcarcinomasofthelungalsoexpressneurofilaments.
      ProductInformation
      FormatSerum
      Control
      • Braintissue
      PresentationRabbitserum.Containsnopreservative.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20oCfromdateofreceipt.
      Applications
      ApplicationDetectNeurofilamentLusingthisAnti-NeurofilamentLAntibodyvalidatedforuseinIC,IH,IH(P)&WB.
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      • WesternBlotting
      ApplicationNotesWesternBlotAnalysis:
      1:1000dilutionofapreviouslotdetectedNEUROFILAMENTLon10μgofmousebrainlysates.

      Immunocytochemistry:
      A1:200-1:500dilutionofapreviouslotwasusedinIC.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenPurifiedporcineNF-L
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      SpecificityRecognizesNeurofilament-Light(NF-L)
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      • Feline
      • Canine
      • Pig
      • Bovine
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • NEFL
      • CMT1F
      • NF-L
      • NF68
      • CMT2E
      • NFL
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P07196#Neurofilamentsusuallycontainthreeintermediatefilamentproteins:L,M,andHwhichareinvolvedinthemaintenanceofneuronalcaliber.
      SIZE:543aminoacids;61517Da
      DOMAIN:SwissProt:P07196Theextramassandhighchargedensitythatdistinguishtheneurofilamentproteinsfromallotherintermediatefilamentproteinsareduetothetailpieceextensions.Thisregionmayformachargedscaffoldingstructuresuitableforinteractionwithotherneuronalcomponentsorions.
      PTM:O-glycosylated(Bysimilarity).
      DISEASE:SwissProt:P07196#DefectsinNEFLarethecauseofCharcot-Marie-Toothdiseasetype1F(CMT1F)[MIM:607734].CMT1FisaformofCharcot-Marie-Toothdisease,themostcommoninheriteddisorderoftheperipheralnervoussystem.Charcot-Marie-Toothdiseaseisclassifiedintwomaingroupsonthebasisofelectrophysiologicpropertiesandhistopathology:primaryperipheraldemyelinatingneuropathyorCMT1,andprimaryperipheralaxonalneuropathyorCMT2.NeuropathiesoftheCMT1grouparecharacterizedbyseverelyreducednerveconductionvelocities(lessthan38m/sec),segmentaldemyelinationandremyelinationwithonionbulbformationsonnervebiopsy,slowlyprogressivedistalmuscleatrophyandweakness,absentdeeptendonreflexes,andhollowfeet.CMT1Fischaracterizedbyonsetininfancyorchildhood(range1to13years).&DefectsinNEFLarethecauseofCharcot-Marie-Toothdiseasetype2E(CMT2E)[MIM:607684].CMT2EisanautosomaldominantformofCharcot-Marie-Toothdiseasetype2.NeuropathiesoftheCMT2grouparecharacterizedbysignsofaxonalregenerationintheabsenceofobviousmyelinalterations,normalorslightlyreducednerveconductionvelocities,andprogressivedistalmuscleweaknessandatrophy.
      SIMILARITY:Belongstotheintermediatefilamentfamily.
      MISCELLANEOUS:NF-Listhemostabundantofthethreeneurofilamentproteinsand,astheothernonepithelialintermediatefilamentproteins,itcanformhomopolymeric10-nmfilaments.
      MolecularWeight68-70kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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