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Millipore/AB5971 | Anti-HuD Antibody/AB5971/100 µg
  • Millipore/AB5971 | Anti-HuD Antibody/AB5971/100 µg

Millipore/AB5971 | Anti-HuD Antibody/AB5971/100 µg

價格: ¥4188.00 市場價: 6980.00

貨號: AB5971
品牌: Millipore
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    • Description
      CatalogueNumberAB5971
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-HuDAntibody
      ProductInformation
      FormatAffinityPurified
      PresentationAffinitypurifiedimmunoglobulin.Precipitatedantibodyinasolutionof50%saturatedammoniumsulfateandPBScontainingnopreservatives.
      StorageandShippingInformation
      StorageConditionsMaintainunopenedvialat-70°Cforupto6months.Avoidrepeatedfreeze/thawcycles.

      PREPARATIONANDUSE:

      Toreconstitutetheantibody,centrifugetheantibodyvialatmoderatespeed(5,000rpm)for5minutestopellettheprecipitatedantibodyproduct.Carefullyremovetheammoniumsulfate/PBSbuffersolutionanddiscard.Itisnotnecessarytoremovealloftheammoniumsulfate/PBSsolution:10μLofresidualammoniumsulfatesolutionwillnotaffecttheresUSPensionoftheantibody.Donotlettheproteinpelletdry,asseverelossofantibodyreactivitycanoccur.

      ResuspendtheantibodypelletinasuitableBIOLOGicalbuffersuchasPBSorTBS(pH7.3-7.5)toafinalconcentrationof1.0mg/mL.Forexample,toachievea1.0mg/mLconcentrationwith50μgofprecipitatedantibody,theamountofbufferneededwouldbe50μL.

      Carefullyaddtheliquidbuffertothepellet.DONOTVORTEX.Mixbygentlestirringwithawidepipettiporgentlefinger-tapping.Lettheprecipitatedantibodyrehydratefor1hourat4-25°Cpriortouse.Smallparticlesofprecipitatedantibodythatfailtoresuspendarenormal.Vialsareoverfilledtocompensateforanylosses.

      Therehydratedantibodysolutionscanbestoredundilutedat2-8°Cfor2monthswithoutanysignificantlossofactivity.Note,thesolutionisnotsterile,thuscareshouldbetakenifproductisstoredat2-8°C.

      Forstorageat-20°C,theadditionofanequalvolumeofglycerolcanbeused,however,itisrecommendedthatACSgradeorhigherglycerolbeused,assignificantlossofactivitycanoccuriftheglycerolusedisnotofhighquality.

      Forlong-termstorageat-70°C,itisrecommendedthattherehydratedantibodysolutionbefurtherdiluted1:1witha2%BSA(fractionV,highest-gradeavailable)solutionmadewiththerehydrationbuffer.Theresulting1%BSA/antibodysolutioncanbealiquotedandstoredfrozenat-70°Cforupto6months.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationDetectHuDusingthisAnti-HuDAntibodyvalidatedforuseinWB,IH.
      KeyApplications
      • WesternBlotting
      • Immunohistochemistry
      ApplicationNotesWesternblot:1:200-1:1,000usingECL.

      Immunohistochemistry:1:200-1:1,000.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BiologicalInformation
      ImmunogenSyntheticpeptide,aminoacids26-42ofHuD.
      HostRabbit
      SpecificityRecognizesHuD.Themolecularweightoftheproteinis41,766Daltons.
      SpeciesReactivity
      • Human
      • Mouse
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • ELAVL4
      • PNEM
      • HUD
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P26378#Mayplayaroleinneuron-specificRNAprocessing.ProtectsCDKN1AmRNAfromdecaybybindingtoits3"-UTR(Bysimilarity).
      SIZE:380aminoacids;41760Da
      SUBUNIT:ComponentofaTAUmRNPcomplex,atleastcomposedofIGF2BP1,ELAVL4andG3BP(Bysimilarity).
      TISSUESPECIFICITY:Brain.
      PTM:MethylationatArg-243byCARM1weakensprotectivebindingtothe3"-UTRofCDKN1AmRNAanddown-regulatesCDKN1Aproteinexpression,therebymaintainingcellsinaproliferativestate.MethylationisinhibitedbyNGF,whichfacilitatesneuriteoutgrowth(Bysimilarity).
      DISEASE:SwissProt:P26378#Paraneoplasticencephalomyelitissensoryneuropathyisassociatedwithsmallcelllungcancerandischaracterizedbydementia,sensoryloss,andotherneurologicaldisABIlities.Itmayresultfromanimmuneresponseprimarilydirectedagainstasmallcelllungtumorantigenwhichismisdirectedagainstsimilarantigensexpressedinbrain.
      SIMILARITY:SwissProt:P26378##BelongstotheRRMelavfamily.&Contains3RRM(RNArecognitionmotif)domains.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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