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Millipore/AB5820-200UL | Anti-Syntaxin 1 Antibody/AB5820-200UL/200 µL
  • Millipore/AB5820-200UL | Anti-Syntaxin 1 Antibody/AB5820-200UL/200 µL

Millipore/AB5820-200UL | Anti-Syntaxin 1 Antibody/AB5820-200UL/200 µL

價格: ¥7524.00 市場價: 12540.00

貨號: AB5820-200UL
品牌: Millipore
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    • Description
      CatalogueNumberAB5820-200UL
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-Syntaxin1Antibody
      ProductInformation
      FormatAffinityPurified
      Control
      • CONTROLANTIGEN:Includedfreeofchargewiththeantibodyis50μgofcontrolantigen.Thestocksolutionoftheantigencanbemadeupusing100μLofPBS.Forpositivecontrol,inWesternblotusing50ngofproteinperMinigellane.Fornegativecontrol,preincubate5-10μgoffusionproteinwith1μgofantibodyforonehouratroomtemperature.Optimalconcentrationsmustbedeterminedbytheenduser.
      PresentationAffinitypurifiedimmunoglobulin.Lyophilizedfromphosphatebufferedsaline,pH7.4,containing1%BSA,and0.025%sodiumazideasapreservative.Reconstitutewith200μLofsteriledeionizedwater.Centrifugeantibodypreparationbeforeuse(10,000xgfor5min).
      StorageandShippingInformation
      StorageConditionsMaintainlyophilizedmaterialat-20°Cforupto12monthsafterdateofreceipt.Afterreconstitutionmaintainat-20°Cinundilutedaliquotsforupto6months.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationDetectSyntaxin1usingthisAnti-Syntaxin1AntibodyvalidatedforuseinWB,IH.
      KeyApplications
      • WesternBlotting
      • Immunohistochemistry
      ApplicationNotesWesternblot:1:1000usingECLonratbrainmembranes.

      Immunohistochemistryonratbrainsections:AB5820isdirectedagainstanintracellularepitope.Thus,aprocedureincludingpermeABIlizationofcellswith0.2%TritonX100isrecommended.Itisrecommendedthatyoustartwithaworkingconcentrationof30μg/mL(1:33).

      DilutionsshouldbemadeusingacarrierproteinsuchasBSA(1-3%)

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenGSTfusionproteincorrespondingtoacytoplasmicpartofratSyntaxin1A(AccessionnumberP32851)(Bennetetal.1992;Inoue&Akagawa1992).
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      SpecificityRecognizesSyntaxin1AandSyntaxin1B.Theepitopedoesnotsharehomologywithanyotherknownproteins.
      SpeciesReactivity
      • Mouse
      • Rat
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummarySynapticvesiclesstoreneurotransmittersthatarereleasedduringcalcium-regulatedexocytosis.Thespecificityofneurotransmitterreleaserequiresthelocalizationofbothsynapticvesiclesandcalciumchannelstothepresynapticactivezone.Syntaxinsfunctioninthisvesiclefusionprocess.SyntaxinsalsoserveasasubstrateforbotulinumneurotoxintypeC,ametalloproteasethatblocksexocytosisandhashighaffinityforamolecularcomplexthatincludesthealpha-latrotoxinreceptor(MIM600565)whichproducesexplosiveexocytosis(Zhangetal.,1995[PubMed7622072]).[suppliedbyOMIM]
      GeneSymbol
      • STX1A
      • HPC-1
      • STX1
      • Syntaxin-1A
      • p35-1
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q16623#Potentiallyinvolvedindockingofsynapticvesiclesatpresynapticactivezones.Mayplayacriticalroleinneurotransmitterexocytosis.
      SIZE:288aminoacids;33023Da
      SUBUNIT:PartoftheSNAREcorecomplexcontainingSNAP25,VAMP2andSTX1A.ThiscomplexbindstoCPLX1.BindsSYTL4andSTXBP6.FoundinaternarycomplexwithSTX1AandSNAP25(Bysimilarity).FoundinacomplexwithVAMP8andSNAP23.InteractswithVAPAandSYBU.
      SUBCELLULARLOCATION:Cellmembrane;Single-passtypeIVmembraneprotein.&Isoform2:Secreted(Probable).
      TISSUESPECIFICITY:Isoform1ishighlyexpressedinembryonicspinalchordandgangliaandinadultcerebellumandcerebralcortex.Isoform2isexpressedinheart,liver,fat,skeletalmuscle,kidneyandbrain.
      DISEASE:SwissProt:Q16623#HaploinsufficiencyofSTX1Amaybethecauseofcertaincardiovascularandmusculo-skeletalabnormalitiesobservedinWilliams-Beurensyndrome(WBS),araredevelopmentaldisorder.Itisacontiguousgenedeletionsyndromeinvolvinggenesfromchromosomeband7q11.23.
      SIMILARITY:SwissProt:Q16623##Belongstothesyntaxinfamily.&Contains1t-SNAREcoiled-coilhomologydomain.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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