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Millipore/AB5454 | Anti-TUC-4 Protein Antibody/AB5454/100 µL
  • Millipore/AB5454 | Anti-TUC-4 Protein Antibody/AB5454/100 µL

Millipore/AB5454 | Anti-TUC-4 Protein Antibody/AB5454/100 µL

價(jià)格: ￥4188.00 市場(chǎng)價(jià): 6980.00
貨號(hào): AB5454
品牌: Millipore
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    • Description
      CatalogueNumberAB5454
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-TUC-4ProteinAntibody
      AlternateNames
      • ULIP-1Protein
      • CRMP-4
      • DRP-3
      • Dihydropyrimidinase-like3
      BackgroundInformationTUC(TOAD[TurnedOnAfterDivision]/Ulip/CRMP)isafamilyofproteinsthathaveemergedasstrongcandidatesforaroleinneuronalgrowthconesignaling.TheTUC-4familymembersreachtheirhighestexpressionlevelsinearlypost-mitoticneuronsduringtheirpeakperiodsofaxonalgrowth.TUC-4isnotexpressedinneuralProgenitorsoradultneurons(withminorexception).DatasuggeststhatTUC-4playsaroleinaxonaloutgrowth,pathfindingandpossIBLyintheprocessthatpermitsgrowingaxonstochooseproperroutesandtargets(Quinn,1999).TheTUC-4proteinisexpressedprimarilywithinthecytoplasmofpostmitoticneuronsastheybegintheirmigrationoutoftheventricularzoneintothedevelopingcorticalplate(Minturn,1995a).TUC-4isexpressedinneuronalcellbodies,neurites,andalongthemostdistalregionsofneuronalgrowthcones,includingthelamellipodiaandfilopodia(Minturn,1995b).ExpressionofTUC-4isobservedbydayE12inratscoincidentwiththeexpressionoftheearlyneuronalMarkerclassIIIbeta-tubulin(Quinn,1999).TUC-4expressionisrarelyobservedinneuronalcellsexpressingthemitoticmarkerPCNA(Minturn,1995a).TheexpressionofTUC-4hasbeenshowntobeupregulatedinresponsetoNGFinrats(Minturn,1995b)andinresponsetoretinoicacidinahumanneuroblastomacellline(SMS-KCNR;Gaetano,1997).
      ProductInformation
      FormatSerum
      Control
      • POSITIVECONTROL:Fetalbrain/spinalcordtissue.HumanneuroblastomacelllineSMS-KCNRorratPC12cells.
      PresentationRabbitSerum.Liquidcontaining0.2%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainfor6monthsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationAnti-TUC-4ProteinAntibodydetectslevelofTUC-4Protein&hasbeenpublished&validatedforuseinIC,IH,IP&WB.
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • WesternBlotting
      ApplicationNotesImmunohistochemistry:1:1,000-1:5,000usingPFA(DABdetection).
      Immunocytochemistry:1:1,000-1:5,000(DABdetection).
      Westernblot:1:2,500-1:25,000usingalkalinephosphatase.
      Immunoprecipitation

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenSyntheticpeptidecorrespondingtoaminoacids499to511ofTUC-4aandaminoacids612-624ofTUC-4b(YDGPVFDLTTTPK)
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      SpecificitySpecificallyrecognizestheTUC-4(Ulip-1/CRMP-4/DRP-3)splicevariants,TUC-4a(64kDa)andtheN-terminallyextendedTUC-4b(75kDa)(Quinn,2003).AB5454hasbeenreportedtolabelearlypostmitoticneuronsinprenatalratbrain.Labelingisobservedprimarilywithinthecytoplasmalongthemostdistalregionsofneuronalgrowthconesincludinglamellipodiaandfilopodia.TheantibodyisnotreactivewiththeproteinsTUC-1(Ulip-3/CRMP-1),TUC-2(Ulip-2/CRMP-2)orTUC-3(Ulip-4/CRMP-3).ItdoesnotlabelneuralprogenitorsorrADIalglia.
      SpeciesReactivity
      • Human
      • Mouse
      • Monkey
      • Rat
      • Feline
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • DPYSL3
      • CRMP-4
      • DRP3
      • CRMP4
      • ULIP
      • DRP-3
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q14195#Mayregulatevesiclefunctioninthegrowthcone(Bysimilarity).
      SIZE:570aminoacids;61963Da
      SUBUNIT:Interactswithsynapticvesicleprotein2andSH3Adomainofintersectin(Bysimilarity).
      SUBCELLULARLOCATION:Cytoplasm(Bysimilarity).Cellprojection,growthcone(Bysimilarity).Note=Colocalizeswithsynapticvesicleprotein2inthecentralregionofthegrowthcone(Bysimilarity).
      TISSUESPECIFICITY:Mainlyinheartandskeletalmuscle.Alsoexpressedstronglyinfetalbrainandspinalcord.
      SIMILARITY:SwissProt:Q14195##Belongstothedehydropyrimidinasefamily.
      MolecularWeight64&74kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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