Immunogen | Syntheticphosphopeptidefromhumanmyosin,smoothandnon-muscleregulatorylightchain. |
Epitope | phospho-specific(Ser19) |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Rabbit |
Specificity | Myosin,human,smoothandnon-muscleregulatorylightchain,phosphoSer19.ByWesternblottheantibodyrecognizesthe~20kDaphosphorylatedprotein.Theimmunogensequencediffersfromthatofthesarcomeric/cardiacformofmyosinregulatorylightchain.Theantibodyrecognizesmonophosphorylated(pSer19)anddiphosporylated(pThr18-pSer19)formsoftheprotein.Reactivitytonon-phosphorylatedhumanmyosin,smoothandnon-muscleregulartorylightchainisminimal(lessthan1%)byELISA. |
SpeciesReactivity | |
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | MyosinisahexamericATPasecellularmotorprotein.Itiscomposedoftwoheavychains,twononphosphorylatablealkalilightchains,andtwophosphorylatableregulatorylightchains.Thisgeneencodesamyosinalkalilightchainexpressedinbothslow-twitchskeletalmuscleandinnonmuscletissue. |
GeneSymbol | |
Modifications | |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P14649#Regulatorylightchainofmyosin.Doesnotbindcalcium. SIZE:208aminoacids;22764Da SUBUNIT:Myosinisanhexamerof2heavychainsand4lightchains. SIMILARITY:SwissProt:P14649##Contains3EF-handdomains. |