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Millipore/AB2033 | Anti-Fibronectin Antibody/AB2033/100 µg
  • Millipore/AB2033 | Anti-Fibronectin Antibody/AB2033/100 µg

Millipore/AB2033 | Anti-Fibronectin Antibody/AB2033/100 µg

價格: ￥4068.00 市場價: 6780.00
貨號: AB2033
品牌: Millipore
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    • Description
      CatalogueNumberAB2033
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-FibronectinAntibody
      BackgroundInformationFibronectinisahighmolecularweightextracellularadhesionmoleculeinvolvedinmanycellularprocesses,includingtissuerepairorwoundhealing,embryogenesis,bloodclotting,andcellmigration/adhesion.Fibronectinexistsintwomajorforms:1)asaninsolubleglycoproteindimerthatservesasalinkerintheextracellularmatrix(ECM),and2)asasolubledisulfide-linkeddimerfoundintheplasma.Theplasmaformissynthesizedbyhepatocytes,andtheECMformismadebyfibroblasts,chondrocytes,endothelialcells,macrophages,aswellascertainepithelialcells.Fibronectinsometimesservesasageneralcelladhesionmoleculebyanchoringcellstocollagenorproteoglycansubstrates.ItcanalsoservetoorganizecellularinteractionwiththeECMbybindingtodifferentcomponentsoftheECMandtomembrane-boundfibronectinreceptorsoncellsurfaces.TheimmunogenforfibronectinpolyclonalantibodyAB2033ispurifiedmouseplasma.Theantibodyshowslessthan0.1%reactivitywithmouselaminin,andcollagentypesI,III,andIVbyrADIoimmunoassay.
      ProductInformation
      FormatAffinityPurified
      Control
      • Extracellularmatrixinmosttissue
      PresentationPurifiedrabbitpolyclonalinbuffercontaining0.01Mphosphate,0.09MNaCl,pH7.2withnopreservatives.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20oCfromdateofreceipt.
      Applications
      ApplicationDetectFibronectinusingthisAnti-FibronectinAntibodyvalidatedforuseinELISA,IC,IH,RIA.
      KeyApplications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Radioimmunoassay
      ApplicationNotesImmunohistochemistry:
      1:80dilutionofapreviouslotwasusedinimmunofluorescentstainingoffrozenmouseskinandlivertissues.

      Radioimmunoassay:
      ApreviouslotofthisantibodywasusedinRIA.

      ELISA:
      A1:200dilutionofapreviouslotwasusedinELISA.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenFibronectinextractedandpurifiedfrommouseplasma.
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      SpecificityThisantibodyreactswithmousefibronectin.Showslessthan0.1%reactivitywithmouseLaminin,andCollagenTypesI,III,andIVbyradioimmunoassay.
      SpeciesReactivity
      • Mouse
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined.
      GeneSymbol
      • FN1
      • MSF
      • FN
      • CIG
      • LETS
      • FINC
      PurificationMethodImmunoAffinityPurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity).
      SIZE:2386aminoacids;262607Da
      SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1.
      SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix.
      TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.
      DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage.
      PTM:Sulfated.
      SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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