Immunogen | An18aminoacidpeptidesequenceneartheNH2-terminusofratbraindopaminetransportercoupledtoKLH(Kiltyetal.1991;Girosetal.1991;Shimadaetal.1991;Kitayma1992).Thisregionispresumablythecytoplasmicdomain. |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Rabbit |
Specificity | RecognizesDopaminetransporter(DAT).ByWesternblottheantibodyrecognizesabroad80kDabandinratstriatum(Lewetal.1992;Freedetal.1995;Vaughanetal.1996)andnotcerebellum.RatDATcontainsvariouscarbohydratemoietiesandneuraminidaseandN-Glycanasetreatmentreducesitsmolecularweighttoabout55kDa. |
SpeciesReactivity | |
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Thedopaminetransporter(DAT1)mediatestheactivereuptakeofdopaminefromthesynapseandisaprincipalregulatorofdopaminergicneurotransmission.TheDAT1genehasbeenimplicatedinhumandisorderssuchasparkinsonism,Tourettesyndrome,andsubstanceabuse(Vandenberghetal.,1992[PubMed1359373]).[suppliedbyOMIM] |
GeneSymbol | |
PurificationMethod | ImmunoAffinityPurified |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:Q01959#Aminetransporter.Terminatestheactionofdopaminebyitshighaffinitysodium-dependentreuptakeintopresynapticterminals. SIZE:620aminoacids;68495Da SUBUNIT:Homooligomer;disulfide-linked.InteractswithPRKCABPandTGFB1I1. SUBCELLULARLOCATION:Membrane;Multi-passmembraneprotein. SIMILARITY:SwissProt:Q01959##Belongstothesodium:neurotransmittersymporter(SNF)family. MISCELLANEOUS:Thisproteinisthetargetofpsychomotorstimulantssuchasamphetaminesorcocaine. |
MolecularWeight | 80kDa |